Tim-3分子表达异常与原因不明复发性流产的关系

Abnormal expression of Tim-3 in unexplained recurrent spontaneous abortion

目的探究T细胞免疫球蛋白粘蛋白分子3(Tim-3)表达异常与不明原因复发性流产(URSA)的关系。方法选择31例URSA患者作为研究组,并以同期30例正常孕妇作为对照组。采用流式细胞术检测两组外周血单个核细胞(PBMC)中CD4+Tim-3+细胞/CD4+细胞的比例,Western Blot技术检测蜕膜中Tim-3蛋白的相对含量,并以免疫磁珠法分选CD4+Tim-3+细胞,荧光定量RT-PCR检测两组外周血PBMC、CD4+Tim-3+细胞及蜕膜组织中Tim-3 mRNA的表达。结果 URSA组中,PBMC中CD4+Tim-3+细胞/CD4+细胞为5.23±0.96%,蜕膜中Tim-3蛋白的相对表达量为0.64±0.09,PBMC及蜕膜中Tim-3 mRNA的相对表达量为0.65±0.09及0.50±0.07,均显著高于正常对照组(P<0.01)。但是,URSA组CD4+Tim-3+细胞(Th1细胞)Tim-3 mRNA的表达量低于正常对照组,差异具有显著性(P<0.01)。结论 Th1细胞Tim-3表达降低,Th1细胞过度活化可能参与了URSA的发生发展过程。

Objective： To explore the relationship between abnormal expression of T eel] immunoglobulin - and muein - domain containing molecular- 3 （Tim- 3 ） and unexplained recurrent spontaneous abortion （URSA）. Methods： 31 pregnant women suffered with URSA were recruited as the study group and 30 normal pregnant women were chosen as the control group. For the two groups, CD4^± Tim - 3 ^± cells/CD4 ^± cell percentage in peripheral blood mononuelear （ PBMC ） were detected by flow eytometry. Then CD4^± Tim - 3^± ceils were collected by magnetic activated cell sorting. The expression levels of Tim - 3 mRNA in PBMC, CD4 ^± Tim - 3 ^± cells and decidua were detected by fluorescent quantitative RT - PCR. Besides, the relative amount of Tim - 3 protein was also analyzed using Western blot. Results ： In URSA group, the CD4 ^± Tim - 3 ^± cells/CD4^± cell percentage in PBMC was 5.23 ± 0. 96% , the relative level of Tim - 3 protein in decidua was 0. 64 ± 0. 09, the relative amounts of Tim - 3 mRNA in PBMC and decidua were 0. 65 ± 0. 09 and 0. 50 ± 0. 07 respectively, which were all significantly higher that those in the control group. However, the expression of Tim - 3 mRNA by CD4^± Tim -3^± ceils （Thl cells） in the URSA group was statistically lower than that in normal control. Conclusion： Down - regulation of Tim - 3 on Thl cells and excessive activation of Thl might contribute to the development of URSA.