rhuIL-2对妊娠中期小鼠外周血清雌激素和孕激素水平的影响

The influence of rhuIL-2 on the estrogen or progesterone levels of mice in mid-gestation

目的探讨不同浓度重组人白介素-2(rhuIL-2)对妊娠中期小鼠外周血清雌激素(E2)、孕激素(P)水平及P/E2比值的影响。方法 BALB/c孕鼠随机分入4组,妊娠第7、8、9天行腹腔药物注射(空白对照组予灭菌水0.2ml,低、中、高剂量组分别予rhuIL-2500U、1KU、2KU),第12天取血,ELISA法测定血清E2、P浓度,计算P/E2比值,并评价妊娠结局。结果与空白组相比,处理组血清E2、P浓度明显降低(P<0.05),不良妊娠结局事件增加(P<0.05),P/E2比值未观察到明显异常(P>0.05)。结论伴随着外周血清E2、P水平的下降,呈不同程度升高的妊娠中期小鼠血清IL-2水平,导致了病理性妊娠结局事件增加,由此推测:妊娠中期小鼠血清IL-2水平增高可能通过干扰血清E2、P水平进而影响正常妊娠。

Objective ： To discuss the influence of different concentrations of rhuIL - 2 on the serum estrogen （ E2 ） , progesterone （P） levels and the P/E2 ratio of mice in mid -gestation. Methods： BALB/c mice were divided into one control group and three treat- ment groups with different dosage of rhuIL - 2. Pregnant mice were injected intraperitoneally by sterile water in control group and rhuIL -2 in treatment groups on the 7th, 8th, 9th day of gestation. After blood collection on the 12tb, serum E2, P and P/E2 ratio were de- tected. Besides, weight increment of body, average embryonic weight, number of the normal and absorption embryo were observed too. Results ： Compared with the control group, the levels of serum E2 and P reduced significantly, and the incidence of adverse pregnancy outcomes increased in three treatment groups, but the P/E2 ratio had no obvious difference. Conclusions ： With the reduction of serum E2 and P levels, the increased incidence of adverse pregnancy outcomes were caused by the increased serum rhuIL - 2 levels in mid - gestation. Thus, it was concluded that normal pregnancy may be influenced by reducing the serum E2 , P levels and the P/E2 ratio under higher levels of IL -2 in maternal serum.