异丙酚控制幼鼠癫痫持续状态疗效及脑保护的研究

The brain protection and effect of propofol used to control her pups of epileptic state

目的探讨异丙酚对Wistar大鼠幼鼠癫痫持续状态的控制疗效及脑保护作用。方法将60只Wistar大鼠随机分为4组,空白对照组,致痫组(异丙酚组,安定组,盐水组)。戊四氮(PTZ)诱导大鼠SE,建立SE模型。分别给予腹腔注射止抽药物,记录各组控制SE的时间(min)。并于止抽后24h、72h、7d断头,取脑,常规病理切片,切取海马组织,染色,光镜及电镜下观察海马结构形态学改变。应用流式细胞术和琼脂糖凝胶电泳,检测海马神经元细胞凋亡,证实异丙酚对脑保护作用和脑神经元细胞凋亡的影响。结果异丙酚组控制癫痫持续时间与地西泮组无显著差异(P>0.05)。异丙酚组神经元细胞大致正常,仅少数出现线粒体肿,细胞结构出现轻微改变。较其他组改变轻微。流式细胞术和琼脂糖凝胶电泳检测可见,异丙酚组神经元细胞凋亡数量较其他组少。结论异丙酚可以有效控制癫痫持续状态。形态学观察说明,异丙酚对脑细胞有一定的保护作用。流式细胞术检测和琼脂糖凝胶电泳实验证实,异丙酚则对神经元凋亡有有效的抑制作用,可以减少神经元的凋亡,保护脑细胞。异丙酚用于癫痫持续状态的治疗,并对SE脑损伤的远期后果有其有效的防治价值。

Objective： To explore the propofol control juenile Wistar rats epileptic state effect and the brain protection function. Methods ： Will Wistar rats were randomly divided into four groups, control and intraoperational group （in the propofol groups, stabili- ty, saline groups）. PTZ induced epileptic state established in rats SE model. To stop hyperspasmia, intraperitoneal drug are injected control epileptic state time record group. Stop hyperspasmia after 24h, 72h, 7d taking brain, conventional pathological sectioning to hippocampal structure, dyeing. On light microscope and electron microscope observe to hippocampal structure morphology change. Application by streaming cell technique or a＊ garose gelelectrophoresis technique, detections hippocanlpal neurons ceils apoptosis, confirmed the propofol in brain tissue protection and nerve cell apoptosis. Results： In the Propofol group control epileptic time and diazepam group had no significant differences （P 〉 0. 05）. It is normal in the Propofol group, neurons arroughly only a handful of mitochondria swelling and cellular structure appears slightchange. A slight change of other groups. Streaming cells and agarose gelelectro- phoresis visibl in the propofol group, the number of apoptosis neurons less than other groups. Conclusions： Propofol can effectively control epileptic state. Morphology explain that propofol in brain tissue certain protective effect. Streaming ceils operation test and agarose gel electrophoresis experiment proved that propofol of apoptosis neurons can effectively reduce the inhibition of apoptosis neurons, protect brain ceils. Propofol for epileptic state treatment, and epileptic state of brain damage longtermconsequences of its effective prevention and value.