应用荧光原位杂交技术检测自然流产组织染色体数目异常

Application of fluorescence in situ hybridization(FISH) in detection of chromosomal numerical abnormalities of spontaneous abortion tissues

为评估荧光原位杂交技术在检测自然流产组织染色体数目异常中的价值和意义,本研究应用FISH技术检测105例自然流产绒毛或胎儿肌肉组织的13、21、18、X、Y染色体的数目,结果显示染色体数目异常的样本为45例,占其中42.86%;数目异常率大于60%的样本为39例;数目异常率介于10%-60%的样本为6例,其中早期流产组织中染色体异常的为29例,占64.44%;常见的数目异常类型为常染色体三体和X单体。与传统的制备染色体技术相比,FISH技术具有快速准确、稳定性高等特点,可以为查明流产原因提供依据,具有很高的临床应用价值。

To assess the clinical practice value of fluorescence in situ hybridization （FISH） in detection of chromosomal numerical abnormalities of spontaneous abortion tissues. Detecting the chromosomal number （13, 21, 18, X, Y） of 105 samples from villous tissue and muscle tissue by FISH, the results displayed that 45 cases showed numerical abnormalities, account for 42. 86% ; the abnormal rate of 36 cases was higher than 60%, the abnormal rate of 9 cases was betweenl0% and 60%, and 29 cases showed numerical abnormalities which were from early spontaneous abortion tissues, account for 64.44%. The most frequent type of numerical abnormalities were trisomy and X monosomy Compared to traditional chromosome preparation method, FISH is more stable and precise, which can provide evidence for finding out the reasons of spontaneous abortion and has great clinical value.