肿瘤坏死因子α-308基因多态性与牙周病相关性

Association of the tumour necrosis factor-α 308 gene polymorphism and susceptibility to severe chronic periodontitis

目的探讨肿瘤坏死因子-α308位点基因多态性与牙周炎易感性的关系。方法采用序列特异引物PCR方法 (SSP-PCR)方法检测了78例重度慢性牙周炎(CP)患者和70例健康对照组的TNFα-308(G/A)位点基因多态性。结果 CP患者TNFα-308各基因型(GG,GA,AA)频率分别为70.5%、0%和29.5%,在对照者中分别为67%,3%和30%二组之间的基因型频率分布的比较,差异没有显著性(χ2=2.29,P>0.05);GG和AA的等位基因频率在CP中分别85.3%和14.7%,在对照者中分别为82%和18%,二者之间比较,差异没有显著性(χ2=0.526,P>0.05)。结论 TNF-α-308基因多态性位点可能不是中国人患牙周病的主要易感位点基因。

Objective： To investigate the association between the TNF-α-308 gene polymorphism and severe chronic Periodontitis（CP）.Methods：The polymorphisms of TNF-α 308 gene site were analysed in 78 Chinese Han CP patients and 70 healthy controls by Sequence – specific primer polymerase chain reaction（SSP-PCR）method.Results： In CP patients TNF-α genotype GG,GA and AA are 70.5%、0%和29.5% respectively,frequency of G and A are 85.3%（GG）and 14.7%（AA）,In healthy controls TNF-α genotype GG,GA and AA are 67%,3% and 30% respectively,frequency of G and A are 82.0%（AA）and 18.0%（GG）,No significant differences were found in the distribution of TNF-α 308 genotypes and allele frequencies between CP patients and controls;Conclusion： Our results indicate that the there may be no association of the tumour necrosis factor-α 308 polymorphism with in periodontal disease Chinese Han population.