神经节苷酯对新生大鼠HIBD脑白质OPC死亡通路阻断作用的研究

To research ganglioside′s obstruct functionary in neonatal rats of OPC′s death channel of alba after hypoxic-ischemic brain damage

目的探讨体外注射神经节苷酯对新生大鼠HIBD后对脑细胞凋亡的影响,以及对脑白质opc死亡通路阻断所体现的脑保护作用。方法新生大鼠30只随机分为:假手术组(n=10)、HIBD组(n=10)、HIBD+神经节苷酯治疗组(n=10).HIBD组和HIBD+神经节苷酯治疗组制作HIBD模型。HIBD+神经节苷酯治疗组每只新生大鼠分别腹腔注射30 mg/kg神经节苷酯,每日1次,连续6d。7日龄时断头取脑,制作脑片,计算每张脑片中OPC的凋亡数目,进行统计学处理,比较组间差异。结果 HIBD后,HIBD组较假手术组OPC的凋亡数目明显增多。具有统计学意义(P<0.01,P=0.0001)HIBD+神经节苷酯治疗组较HIBD组OPC的凋亡数目明显减少,具有统计学意义(P<0.01,P=0.0005)。结论HIBD后新生大鼠OPC的凋亡数目明显增多。神经节苷酯早期干预后可明显减少HIBD后新生大鼠OPC的凋亡数目。从而证明神经节苷酯对新生大鼠HIBD脑白质OPC死亡通路的阻断作用具有一定的效力。

Objective： To approach brain cell apoptotic effect after HIBD of in vitro injection ganglioside to neonate rat,and to reflect brain cell protective effect though obstruct alba opc death passage way.Methods： Thirty neonate rats were randomized into sham operated group（n = 10）、 HIBD group（n = 10） and group（n = 10）.HIBD model was established in the HIBD group and HIBD plus ganglioside group.In HIBD plus ganglioside group,every rat was given ganglioside 30 mg/kg,one time a day.Seven days later,decapitation to encephalon,and to make brain slice.To account apopotosis number of oligodendro-cyte progenitor cells in ecery brain slice and analyzed by statistics.Results： HIBD group were much more than those of sham operation group in apopotosis number of oligodendro-cyte progenitor cells after HIBD.It had statistical significance（P0.01,P=0.0001）.HIBD plus ganglioside group were much shorter than that of HIBD group（P0.01,P=0.0005）.Conclusion： The apopotosis number of oligodendro-cyte progenitor cells of neonate rats were more after HI.Ganglioside could reduce the effect of HI.