血清TNF-α及sHLA-G水平与习惯性流产的关联性分析

The study of relationship between the quantity of soluble forms of human leukocyte antigen-G(sHLA-G),tumor necrosis factor-α and recurrent spontaneous abortion

目的通过定量方法研究测定习惯性流产(RSA)患者血清中的肿瘤坏死因子-α(TNF-α)及可溶性人白细胞抗原G(sHLA-G)的水平,探讨TNF-α及sHLA-G与习惯性流产有无相关性。方法选择早期妊娠孕妇100例,正常对照组:无自然流产史早期妊娠健康孕妇42例,RSA组:有习惯性流产史二次及二次以上的早期妊娠妇女58例,采用酶联免疫(ELISA)法定量检测两组样本血清中TNF-α及sHLA-G的水平。结果 RSA组血清中外周血淋巴细胞的TNF-α表达水平则明显高于正常妊娠组差异有显著性(P<0.05),而sHLA-G的表达水平明显低于正常对照组,差异有显著性(P<0.05)。结论妊娠早期孕妇血清中TNF-α及sHLA-G与妊娠成功有直接关系,TNF-α升高及sHLA-G降低可能会导致习惯性流产的发生。

Objective： To determine the levels of Soluble Forms of Human Leukocyte Antigen -G （sHLA -G）, Tumor Necrosis Factor - a in human serum of patients with recurrent spontaneous abortion to explore relationship between sHLA - G and TNF - a and recurrent spontaneous abortion. Methods： A hundred women in early pregnancy were selected and divided into two groups： 58 in the RSA group and 42 in the control group who had at least one live birth without history of spontaneous abortion. The level of sHLA - G and TNF - a were detected by Enzyme - Linked Immuno Sorbent Assay. Results ： The levels of Soluble Forms of Human Leukocyte Antigen- G （sHLA- G） in human serum of patients with recurrent spontaneous abortion were significantly decreased than those of normal early pregnant women. It had a significant difference （ P 〈 0.05 ）, while the levels of Tumor Necrosis Factor - a by peripheral blood mononuclear cells were significantly higher. It reached a significant difference （P 〈 0. 05 ）. Conclusion ： Both levels of sHLA - G and TNF -a are correlated with the results of pregnancy. The lack of sHLA - G and the excess of TNF - a in human serum may lead to the recurrent spontaneous abortion.