荧光光谱法快速测定银翘片中对乙酰胺基酚

Rapid Fluorospectrophotometric Determination of Acetaminophenol in "Yinqiao" Tablets

在pH 8.6的磷酸盐缓冲溶液中,吡咯红与一定浓度的十二烷基苯磺酸钠(SDBS)发生荧光增强反应,当在该体系中加入对乙酰胺基酚(APAP)后,于激发波长538nm,发射波长558nm处荧光强度明显降低,且降低程度与对乙酰胺基酚的加入量呈线性,方法的线性范围在0.048～1.44mg.L-1之间,检出限(3S/N)为0.048mg.L-1。方法用于银翘片中对乙酰胺基酚的测定,回收率在103.8%～105.4%之间,测定值的相对标准偏差(n=5)在1.8%～4.2%之间。

Abstract： In a phosphate buffer solution of pH 8. 6, the fluorescence intensity of pyrrole-red was enhanced by its reaction with definite amount of sodium dodecylbenzene sulfonate （SDBS）. But when microamount of acetaminophenol was added to this reaction system, significant decrease in fluorescence intensity was observed as measured at the wavelengths of 538 nm （λex） and 558 nm （λcm）. Linear relationship between the magnitude in decrease of fluorescence intensity and mass concentration of acetaminophenol was obtained in the range of 0. 048-1.44 mg ·L^-1 with detection limit （3S/N） of 0. 048 mg·L^-1 The proposed method was applied to the determination of acetaminophenol in “Yinqiao” tablets, giving values of recovery in the range of 103.8%- 105.4%and RSD＇s （n=5） in the range of 1.8%-4.2%