催化荧光光度法测定痕量过氧化氢

Catalytic Fluorophotometric Determination of Trace Amount of Hydrogen Peroxide

在pH 7.0的磷酸氢二钠-磷酸二氢钾缓冲溶液中,四羧基铁酞菁(FeTCPc)能催化过氧化氢氧化L-酪氨酸,形成荧光二聚体,据此提出了荧光光度法测定水中过氧化氢的方法。在激发波长(λex)320nm与发射波长(λem)410nm处,反应体系的ΔF(F-F0)与过氧化氢的浓度在7.6×10-7～2.7×10-5 mol.L-1之间呈线性关系,方法的检出限(3s/k)为5.2×10-8 mol.L-1。以此方法对两种水样进行分析,测得回收率在96.5%～96.9%之间,测定值的相对标准偏差(n=5)在1.0%～2.8%之间。

Based on the catalytic action of tetracarboxyphthalocyanine （FeTCPc） on the oxidation of L-tyrosine by H20z in Naz HPO4-KH2PO4 buffer medium, forming fluorescence dimer, a {luorophotometric method for determination of H2 02 was proposed. Linear relationship was found between values of △F （F-F0） measured at wavelengths of 320 nm （λex） and 410 nm （λem） and concentration of H2O2 in the range of 7.6×10^-7-2.7×10^-5mol·L^-1. Limit of detection （3s/k） of the method found was 5.2×10^-8mol·L^-1 The proposed method was used in the determination of H2O2 in water samples, giving values of recovery ranged from 96.5%-96.9% and values of RSD＇s （n=5） ranged from 1.0%-2.8%