高压消解-原子荧光光谱法测定牛初乳中硒的研究

High Pressure Digestion-Atomic Fluorescence Spectrometric Determination of Selenium in Bovine Colostrum

建立了用氢化物原子荧光法测定牛初乳中的硒,考察了前处理方法和原子荧光仪器对实验结果的影响,最后确定了消解试剂6:2::1的硝酸:过氧化氢:超纯水,用高压消解法处理牛初乳样品,消解温度130-140℃,时间2-3小时;原子荧光的还原剂为14g/l的硼氢化钾,载流液为5%的盐酸。得到硒的检出限0.1ng/ml,相对标准偏差为0.16%-1.61%,回收率范围为88%-102%。

Established by hydride atomic fluorescence method for the determination of selenium in bovine colostrum, investigates the methods of pretreatment and atom fluorescence instrument effects on the experimental results, and finally determine the digestion reagent6： 2： ： 1： nitrate hydrogen peroxide： ultrapure water, with high pressure digestion of bovine colostrum samples, digestion temperature 130-140C, time 2-3hours; atomic fluorescence reducing agent forl4g / L potassium borohydride, load flow solution for 5% hydrochloric acid. Get the selenium detection limit of0.1ng / ml, the relative standard deviation for0.!6%-1.61%, recovery rate range for 88%-102%.