妊高征患者抗中性粒细胞胞浆抗体的检测

Antineutrophil cytoplasmic antibodies(ANCA) in patients with pregnancy induced hypertension(PIH).

目的 探讨抗中性粒细胞浆抗体 (ANCA)在妊高征患者内皮细胞损伤中的作用及可能机理。方法 采用间接免疫荧光法 (IIF)和酶联免疫吸附试验 (ELISA)对 32例中、重度妊高征患者作血中ANCA的定性和定量检测 ,并以 44例正常孕妇作对照。结果 32例妊高征患者ANCA阳性者 13例 (胞浆型ANCA阳性 9例 ,核周型ANCA阳性 4例 ) ,正常孕妇无一例ANCA阳性。妊高征患者ANCA含量 (0 45± 0 18) g/L ,正常孕妇为(0 2 3± 0 0 9) g/L ,两组间定性及定量均有显著性差异 (P <0 0 5 )。

Objective To evaluate the possible role of ANCA in the endothelial cell damage of PIH.Methods ANCA of 32 PIH were investigated by indirect immunofluorescence(IIF) and enzyme-linked immunosorbent assays(ELISA).44 normal pregnancy women served as control.Results In 32 PIH,ANCA positive were in 13 (9 C-ANCA positive, 4 P-ANCA positive). The average level of ANCA in PIH was (0 45±0 18)g/L.There was no positive stainning in the control group and the ANCA concentration was (0 23±0 09)g/L.The level of ANCA in PIH were significantly higher than that of normal pregnancy women.Conclusion ANCA may play a role in the mechanism of endothelial cell damage in PIH.