地塞米松对MS的IFN-γ及IL-10分泌性T细胞的影响

Effect of Dexamethsone on levels of IFN-γ and IL-10 secretory T cell in MS in vitro

目的 检测多发性硬化 (MS)患者外周血单个核细胞在地塞米松 (Dex)影响下的 IFN- γ和 IL- 10的分泌细胞水平。方法 采用酶联免疫斑点技术 (EL ISPOT)检测体外培养的外周血单个核细胞 (MNC)在 CNS髓鞘素抗原 MBP刺激下的地塞米松对照试验 ,检测 IFN -γ和 IL - 10分泌性 T细胞水平 ,并与其他神经疾病 (OND)组及健康对照组的检测结果进行对比。结果 显示 MS患者 IFN- γ分泌细胞水平高于对照组 ,Dex使 MS患者 IFN- γ分泌细胞减少 ,对 IL- 10分泌细胞无明显影响。结论 MS患者存在 Th1/ Th2细胞因子的失衡 ,Dex能抑制 MS Th1类细胞因子 IFN- γ,其治疗作用可能与此有关。

Objective To examine the effect of glococorticids (GCs) on the production of the immunosuppressor cytokine interleukin 10 (IL 10) and the inflammatory cytokine interferon γ(IFN γ) in MS. Method Blood samples from MS patients suffering an acute relapse were obtained. Levels of IL 10 and IFN γ secreting cells in PBMC were determined by ELISPOT. The expressions of IL 10 and IFN γ were studied in blood MNC of the MS patients after being cultured in the presence of myelin basic protein(MBP). Results In vitro experiments employing normal PBMC showed that Dexamethsone downregulated both MBP induced IFN γ and spontaneous expression of IFN γ,no effect on IL 10 secreting cells was found. Conclusion In summary,our results demonstrate a Th1 type cytokine bias in peripheral blood mononuclear cells of untreated MS patients that is reversed by Dex treatment. These findings provide a basis for immune monitoring of patients with MS and suggest that treatments downregulate IFN γ may prove to be beneficial in progressive MS.