整合素Ⅱb/Ⅲa抑制剂抗涎腺腺样囊性癌转移的实验研究

Antimetastatic effect of integrinⅡb/Ⅲa inhibitors on salivary adenoid cystic carcinoma

目的 :探讨涎腺腺样囊性癌 (salivaryadenoidcysticcarcinoma ,SACC)转移与SACC细胞 血小板粘附的关系 ,观察整合素Ⅱb/Ⅲa抑制剂抗SACC转移的作用。方法 :体外实验观察高转移潜能的SACC LM细胞、非高转移潜能的SACC 83细胞与血小板的粘附 ,阿斯匹林、阿斯匹林镁、精氨酸 天冬氨酸 (arginina aspartate ,RD)对SACC LM细胞与血小板粘附的影响 ;动物实验观察阿斯匹林、阿斯匹林镁、RD对SACC LM细胞实验性肺转移的作用。结果 :SACC LM细胞与SACC 83细胞相比和血小板的粘附增高 ,阿斯匹林、阿斯匹林镁、RD在 1,5 ,2 5 μg/ml时抑制血小板与SACC LM细胞的粘附 ,RD能抑制SACC LM实验性肺转移的形成。结论 :SACC的转移与血小板 肿瘤细胞的粘附有关 ,RD能明显抑制SACC的转移。

Objectives:To Study the relationship between metastatic potential of salivary adenoid cystic carcinoma (SACC)and tumor cell platelet adhesion,and to evaluate antimetastatic effect of integrin Ⅱb/Ⅲa inhibitor on SACC Methods:Tumor cell platelet adhesion of highly metastatic SACC LM, non metastatic SACC 83 and effect of aspirin, arginine aspartate (RD), magnesium acetylsalicylate on adhesion were studied in vitro Antimetastatic effect of aspirin, RD,magnesium acetysalicylate on experimental metastasis of SACC was observed in vivo Results:The tumor cell platelet adhesion was significant in SACC LM than in SACC 83;aspirin, RD, magnesium acetylsalicylate inhibitet the adhesion of SACC LM in the final concentration of 1, 5 ,25 μg/ml respectively RD inhibited experimental metastasis of SACC Conclusion:Metastasis of SACC is related to platelet tumor cell adhesion, and RD can inhibit metastasis of SACC