抑癌基因nm23-H_1诱导癌细胞凋亡及抗氧化作用的研究

nm23-H_1 gene induces apoptosis and improves the antioxidation in tumor cell

目的 :初步探讨nm2 3 H1基因的抑癌机制。方法 :实验将nm2 3 H1基因转化进入人喉癌细胞Hep 2 ,使其在细胞中稳定表达。使用电镜观察细胞形态学变化 ,并测定抗氧化剂还原型谷胱苷肽 (GSH)浓度。结果 :nm2 3 H1转化的Hep 2细胞有核固缩、核分离等典型的细胞凋亡现象 ,其GSH浓度也有所升高 (16 9% )。结论 :表明nm2 3 H1具有诱导肿瘤细胞凋亡 ,提高肿瘤细胞抗氧化能力的作用。

Objective:To investigate the effect of the human tumor suppressor gene nm23 H 1 Method:The human tumor suppressor gene nm23 H 1 was transfected in human laryngeal carcinoma cell Hep 2, and obtained the stable expressing cell line Results:Under the electron microscopy, the nuclear condensation and nuclear separation were abserved in the nm23 H 1 transfected Hep 2 cell Moreover, the antioxidant GSH concentration in nm23 H 1 transfected cell was higher than that in control cell Conclusion:The results suggest that the nm23 H 1 gene might cause apoptosis of tumor cell and improve the antioxidation of the tumor cell