膀胱肿瘤细胞凋亡及其对预后的影响

Cellular apoptosis and its influence on prognosis of bladder cancer

目的 :探讨细胞凋亡与膀胱癌临床生物学行为的关系。方法 :采用末端脱氧核苷酸转移酶介导缺口末端标记法 (TUNEL)进行膀胱癌细胞凋亡的检测。结果 :49例膀胱癌标本共有 43例 (87 76 % )发生细胞凋亡 ,细胞凋亡指数为 (45 6 6± 12 49) % ,细胞凋亡指数大小与病人年龄、性别、肿瘤大小、数目无关 (P >0 0 5 ) ,与肿瘤分级、分期呈负相关 (P <0 0 1) ,即肿瘤“瘤级”和“瘤期”越高 ,则细胞凋亡越少 ,易复发肿瘤其细胞凋亡指数明显小于不复发肿瘤 (P <0 0 5 )。结论 :膀胱癌细胞凋亡与肿瘤分级、分期及预后有关。膀胱癌细胞凋亡减少提示肿瘤分化差、易浸润 ,且病人预后较差。

Objective:To explore the relationship between cellular apoptosis and clinicbilogical acting for bladder cancers Methods:The cellular apoptosis of bladder cancers were detected by TUNEL (terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling) assay in 49 cases Results:Cellular apoptosis exhibited in 46 (87 76%) cases and the apoptosis index (AI) was (45 66±12 49)%(±s) for 49 cases of bladder cancers The “AI”of bladder cancer was not related with patients age, sex, size or number of the tumor (P >0 05), but it was related with “grade”and “stage”of the tumor (P< 0 01), specifically the higher “grade”or “stage”the less cellular apoptosis The “AI”in easily recurrent bladder tumors were less than that of primary bladder tumors (P< 0 05) Conclusions:The “AI”of bladder cancer is related with the “grade”, “stage”and prognosis of the tumor A reduced “AI”in bladder cancer indicates a worse differentiated or/and advanced tumor and the detection of cellular apoptosis of bladder cancer is helpful for evaluating the prognosis of the patient