CD44V_6和E-CD的表达与胃癌生物学特性的关系

The relation of CD44V_6 and E-cadherin expressions to the biological behaviour of gastric cancer

目的 探讨CD44V6(CD44splicevariantV6)和E CD (E cadherin)表达与胃癌发生发展的关系 ,评价CD44V6和E CD在胃癌诊断、浸润转移潜能及预后判断中的作用。方法 采用SP免疫组化染色方法 ,检测 2 0例正常胃粘膜上皮 ,43例异型增生和 10 1例胃癌组织的CD44V6和E CD的表达情况。结果 正常胃粘膜CD44V6呈阴性表达 ,异型增生粘膜CD44V6阳性表达率为 30 % (13/4 3) ,胃癌阳性率为 74% (75 /10 1) ,胃癌CD44V6表达阳性率及阳性强度均显著高于异型增生 (P <0 0 5 ) ,CD44V6表达强度与胃癌浸润型生长、淋巴结转移、脉管浸润、浸润深度密切相关 (P <0 0 5 ) ,胃癌Ⅰ、Ⅱ期表达强度低于Ⅲ、Ⅳ期 (P <0 0 5 ) ,CD44V6强阳性者 1、3、5年生存率分别为 71% (17/2 4)、46 % (11/2 4)和 42 % (10 /2 4) ,显著低于弱阳性或阴性表达 ;正常胃粘膜和异型增生组织E CD呈强阳性表达 ,88 1% (89/10 1)的胃癌组织E CD表达减弱 ,E CD表达减弱与胃癌细胞分化差、浸润型生长显著相关 (P <0 0 5 )。E CD表达正常的胃癌病人术后 3、5年生存率显著高于减弱者 (P <0 0 5 ) ;CD44V6表达与E CD表达无显著相关性 (P >0 0 5 )。结论 CD44V6和E CD表达与胃癌发生发展密切相关 ,检测CD44V6和E CD表达有助于胃癌的早期诊断和转?

Objective[WT5”BZ] To elucidate the expression of CD44 splice variant V 6 (CD44V 6) and E cadherin (E CD) in carcinogenesis and progression of gastric cancer, and evaluate its value for early diagnosis and the assessment of tumor metastatic potential and prognosis. [WT5”HZ]Methods[WT5”BZ] The expressions of CD44V 6 and E cadherin of gastric mucosa were detected in 20 cases of healthy control, 43 cases of dysplasia and 101 cases of carcinoma by using streptavidin peroxidase immunohistochemistry.[WT5”HZ] Results[WT5”BZ] The normal gastric mucosa did not express CD44V 6 protein, whereas the positive rate of CD44V 6 expression was 30% (13/43) and 74 3% (75/101) in dysplastic and carcinomatous mucosa respectively. The positive rate of CD44V 6 expression in cancer was significantly higher than that of dysplasia ( P <0 05). The staining intensity of CD44V 6 was siginficantly correlated with the gastric cancer inflitrative growth pattern, lymph node metastasis, vessel invasion and depth of invasion ( P <0 05). The staining intensity in the stages of Ⅰand Ⅱ was significantly lower than that of tumors in stages of Ⅲ and Ⅳ ( P <0 05). The overall survival rate of patients with tumor of strong CD44V 6 expression was significantly lower than that of patients with weak or negative expression. The normal and dysplastic gastric mucosa strongly expressed E cadherin, whereas 88 1% (89/101) gastric cancer showed reduced or absent expression of E cadherin. The significant correlation was observed between low E cadherin expression and cellular dedifferentiation and infiltrative growth pattern ( P <0.05). The three and five year's survival rate of patient with normal E cadherin expression was significantly higher than that of patients with low or negative expression ( P <0 05).[WT5”HZ] Conclusion[WT5”BZ] The expressions of CD44V 6 and E cadherin may be linked with gastric carcinogenesis and progression. Determination of CD44V 6 and E cadherin will help in predicting tumor metastatic potential and prognosis of patients with gastric cancer. [WT5”HZ]