外周血单个核细胞尼克酰胺腺嘌呤二核苷酸磷酸氧化酶与急性心肌梗死左室重构的关系研究

Study of the relationship between nicotinamide adenosine dinucleotide phosphate oxidase in peripheral blood mononuclear cell and left ventricular remodeling in patients with acute myocardial infarction

目的探讨急性心肌梗死（AMI）患者外周血单个核细胞尼克酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶活性与左室重构的关系。方法选择2010年12月至2011年6月住院治疗的52例AMI患者作为AMI组，选择同期住院的25例阵发性室上性心动过速患者作为对照组。行超声心动图检查并计算左室质量指数（LVMI）。人院第2天采空腹静脉血检测生化指标、丙二醛（MDA）水平及NADPH氧化酶活性，并分析MDA水平、NADPH氧化酶活性与LYMI的相关性。结果AMI组MDA水平[（6．14±2．80）μmol／L]较对照组[（2．95±1．82）μmo]]L]显著增高（P〈0．01），AMI组O2-水平[（21．61±10．66）RLU／mg蛋白]较对照组[（7．12±4．48）RLU／mg蛋白]显著增高（P〈0．01），AMI组NADPH氧化酶活性[（2．84±1．05）RLU／mg蛋白]较对照组[（1．97±0．80）RLU／mg蛋白]显著增高（P〈0．01），MDA水平与LVMI呈正相关（r=0．57，P〈0．01），NADPH氧化酶活性与LVMI亦呈正相关（r=0．71，P〈0．01）。结论AMI患者外周血单个核细胞NADPH氧化酶活性增加，在AMI后左室重构中起重要作用。

Objective To investigate the relationship between the activity of nicotinarnide adenosine dinueleotide phosphate（ NADPH ） oxidase in peripheral blood mononuelear cell and left ventrieular remodeling in patients with acute myocardial infarction （AMI）. Methods Fifty-two patients with AMI hospitalized from December 2010 to June 2011 were selected as AMI group, and 25 patients with paroxysmal supraventricular tachyeardia hospitalized in the same period were selected as control group. All patients were examined by Doppler echocardiography and left ventricular mass index （LVMI） was calculated. Fasting blood was drawn on the second day after hospitalization to measure the level of biochemical indexes, malondialdehyde（MDA ） and the activity of NADPH oxidase. The correlation of the level of MDA, the activity of NADPH oxidase and LVMI was analyzed. Results The level of MDA in AMI group was significantly higher than that in control group [ （6.14 ± 2.80）μmol/L vs. （2.95 ± 1.82）μmol/L, P 〈 0.01 ]. The level of 02- in AMI group was also obviously higher than that in control group [ （21.61 ± 10.66） RLU/mg protein vs. （7.12 ± 4.48 ） RLU/mg protein, P 〈 0.01 ], as well as the activity of NADPH oxidase [ （2.84 ± 1.05 ） RLU/mgprotein vs. （1.97 ± 0.80） RLU/mg protein,P 〈 0.01 ]. Positive correlation was found between LVMI and the level of MDA （r = 0.57, P 〈 0.01 ）, as well as between LVMI and the activity of NADPH oxidase （r = 0.71, P 〈 0.01 ）. Conclusions The activity of NADPH oxidase in peripheral blood mononuclear cell increases in AMI patients and plays an important role in left ventricular remodeling after AMI.