谷胱甘肽-S-转移酶多克隆抗体免疫金测定法检测日本血吸虫循环抗原的研究

DETECTION OF CIRCULATING ANTIGEN IN SERA FROM SCHISTOSOMIASIS JAPONICA BY IMMUNO-GOLD ASSAY WITH POLYCLONAL ANTIBODIES AGAINST RECOMBINANT Sj26GST ANTIGEN

目的探讨谷胱甘肽－Ｓ－转移酶（ｒＳｊ２６ＧＳＴ）多克隆抗体诊断日本血吸虫病的应用价值。方法以抗ｒＳｊ２６ＧＳＴ多克隆抗体力捕获并检测抗体，建立双抗体夹心斑点免疫金测定技术，用于检测日本血吸虫循环抗原，并以斑点免疫金测定法和ＥＬＩＳＡ检测抗体作对照。结果３种方法检测急性血吸虫病的敏感性均为１００％；对慢性血吸虫病的敏感性分别为７６．４％、９８．１％和９６．２％；特异性分别为９５．０％、９８．０％和９６．０％。结论ｒＳｊ２６ＧＳＴ抗原对日本血吸虫病具有诊断价值，可望有较好的应用前景。

Objectives To study the value of diagnosis for schistosomiasis japonica by gold immuno-assay with polyclonal antibodies against recombinant Schistosoma japonicum 26 kilo-dalton glutathione s-transferase (rSj26GST) antigen. Methods Polyclonal antibodies against rSj26GST were used as capture and label antibodies, and double antibodies sandwich dot immuno-gold filter assays (S-DIGFA) was developed for detecting circulating antigen of Schistosoma japonicum. Two assays, dot immuno-gold filter assay (SEA-DIGFA) and ELISA(SEA-ELISA) detecting antibodies against SEA, were compared with S-DIGFA. Results The positive rates of these three methods were all 100% for acute schistosomiasis. And for chronic schistosomiasis, the positive rates were 74. 6%, 98. 1 % and 96. 2%,respectively. The negative rates of these three methods for normal persons were 95. 0%,98. 0% and 96. 0%, respectively. Conclusion The method with polyclonal antibodies against rSj26GST showed potential value for diagnosis of schistosomiasis japonica.