ERβ基因表达及多态性与胃癌发生发展的相关性研究

Correlation between ERβ gene polymorphisms and the risk of gastric cancer

目的:研究ERβ基因表达及多态性与胃癌发生发展的相关性。方法:以100例原发性胃癌为实验组,90例胃溃疡为对照组,免疫组织化学SP法检测ERβ蛋白,限制性酶切法检测ERβ基因CA重复序列多态性和G1082A多态性。结果:ERβ实验组和对照组阳性率分别为68.0%和23.3%,差异有统计学意义(P<0.01),并且分化程度低、浸润深、有淋巴结转移和5年死亡者的ERβ阳性率分别高于分化程度高、浸润浅、无淋巴结转移和5年生存者,且Ⅰ、Ⅱ期低于Ⅲ期(P<0.01,P<0.05),而与初复发、年龄、肿瘤大小和组织学类型无关(P>0.05)。ERβ基因CA重复序列对照组和实验组基因型分布均符合Hardy-Weinberg平衡(P>0.05),两组基因型SS、SL、LL分布和等位基因S、L频率的比较,差异无统计学意义(P>0.05)。ERβG1082A多态性中,RsaⅠ和AluⅠ酶切分析:实验组和对照组均符合Hardy-Weinberg遗传平衡定律(P>0.05)。两组基因型rr、Rr、RR分布和等位基因r、R和a、A频率的比较差异无统计学意义(P>0.05);而实验组aa基因型频率与对照组相比,差异有统计学意义(P<0.05),且该基因型与肿瘤的临床分期、分化程度、浸润深度、转移情况、5年生存情况密切相关(P<0.05),与肿瘤初复发、患者年龄、肿瘤大小、组织学类型无关(P>0.05),而等位基因频率中仅a/A与肿瘤的分化程度、浸润深度密切相关(P<0.01)。结论:ERβ与胃癌的发生、分化程度、浸润深度、转移及5年存活率密切相关,并且ERβ的aa基因型和等位基因a/A与胃癌的关系极大。

Objective：To investigate whether ERβ gene expression and polymorphisms can modify the risk of gastric cancer. Methods：Total 100 cases of primary gastric cancer were enrolled as the test group ,and 90 cases of gastric ulcer as the control group. Immunohistochemical SP method was used to detect ER[3 protein,RT-PCR method to detect the polymorphisms of CA repeat sequence and G1082A in ER[3 gene. Results：Positive expression rates of ERβ were 68.0% and 23.3% respectively in the test group and the control group,which had significant differences between two groups （P 〈 0.01）. The positive ERβ expression rates of the subjects with low differentiation degree,high invasion depth,lymph node metastasis and five years death were higher than those with high differentiation degree, shallow invasion depth, without lymph node metastasis and five years survival. And the positive expression rate of ERβ in clinical stages of I and H were significantly lower than that in stage m （P 〈 0.01 ,P 〈 0.05 respectively）. ERβ expression had nothing to do with onset recurrence, age, tumor size, and histological types （P 〉 0.05）. Genotype distribution accords with the Hardyeinberg（H-W） genetic balance in CA repeat sequence of ERβ of gastric cancer group and control group（P 〉 0.05）; There was no siginifican difference in comparative genotype distribution of SS, SL, LL and allele frequency of S and L （P 〉 0.05）. In G1082A polymorphism of ERβ,analysis result of Rsa [ and A lu I digestion showed that two groups were accord with the H-W genetic balauce（P 〉 0.05）;There was no significant difference in comparative genotype of rr,Rr,RR and genotype frequency of r,R/a,A（P 〉 0.05） ;but genotype frequency of aa in the test group was higher than that in the control group （P 〈 0.05）. Genotype in G1082A polymorphism of ERβ was closely related to clinical stages,differentiation degree, infiltration depth,metastases situation and the fiveyear survival condition （P 〈 0.05）,and it wasn＇t related to onset recurrence,age,tumor size and histological types （P 〉 0.05）. Onlyfrequency of a/A was closely related to differentiation degree and infiltration depth of gastric cancer （P 〈 0.01）. Conclusion：ERβ polymorphism has close correlation with clinical stages, differentiation degree,infiltration depth, metastases situation and the five-year survival condition in gastric cancer,and genotypes of aa,alleles of a/A in ERβ are critical factors in gastric cancer.