丹参对大鼠肝脏低温保存损伤的保护作用

Protective effect of salvia miltiorrhiza on cryopreservation injury of rat liver

目的 研究丹参对大鼠肝脏低温保存损伤的保护作用。方法 采用大鼠肝脏离体非循环灌注模型 ,观察乳酸林格液 (LR液 )中加入不同剂量丹参后保存大鼠肝脏 1 2h的效果。结果 丹参组 ( 60 0mg/g)肝组织ATP含量 ( 6.0 8± 0 .67) μmol/g及分泌胆汁量 ( 1 0 5.6± 1 2 .4) μl/h明显高于对照组 ( 2 .52± 0 .31 ) μmol/g及 ( 57.4± 8.2 ) μl/h(P <0 .0 5) ,丹参组 ( 60 0mg/g)肝组织 2 ,3 二羟苯甲酸 ( 2 ,3 DHBA) ( 0 .1 54± 0 .0 1 3)nmol/g、2 ,5 DHBA( 1 .354± 0 .0 68)nmol/g及流出液天门冬氨酸转氨酶 (AST) ( 38.4± 3.7)U/L明显低于对照组 ( 0 .2 4 5± 0 .0 2 1 )nmol/g、( 2 .1 0 5± 0 .0 97)nmol/g及( 76.4± 9.2 )U/L ,差异有显著性 (P <0 .0 5)。结论 丹参可明显提高供体肝脏的保存效果 。

Objective\ To study the protective effects of salvia miltiorrhiza on cryopreservation injury of rat liver. Methods\ Using isolated nonrecirculated perfusion rat liver model, the preservation effects of Lactate Ringer's (LR) solution containing salvia miltiorrhiza with different doses were compared. Results \ After the rat livers were preserved for 12 h in LR solution with the addition of salvia miltiorrhiza, the contents of ATP, TAN, AEC and bile production were significantly higher than in the control group ( P <0.05). In a certain range of the dosage of salvia miltiorrhiza, the contents of ATP, TAN, AEC and bile production were positively correlated with the dosage in the LR solution. It was also found that salvia miltiorrhiza had a marked protection against hydroxyl free radical and alleviated the injury on liver cells.Conclusion\ Salvia miltiorrhiza could obviously improve the preservation effects of donor liver. The mechanism might be that it could improve the energy metabolism of cryopreservation liver and play a role against free radical.\;