CTLA4Ig基因转染对大鼠胰岛移植的影响

Role of CTLA4Ig gene in transplantation of pancreatic islets in rats

目的 研究CTLA4Ig基因在糖尿病大鼠体内表达及其产物延长胰岛移植物存活的作用。方法 利用Lipofectin载体包裹CTLA4IgcDNA质粒后转染鼠胰岛和肌肉细胞 ,检测移植后CTLA4Ig基因表达水平 ,观察CTLA4Ig基因表达在延长糖尿病鼠胰岛移植物和大鼠存活时间的作用。结果 A组胰岛移植第 7天 2只大鼠血清CTLA4Ig呈阳性 (阳性率 2 0 % ) ,分别为 14ng ml和 31ng ml。B组胰岛移植后维持血糖正常时间平均 (3 .6± 5 .1)d ,A组胰岛移植后维持血糖正常平均 (14 .8± 12 .3)d ,两组比较差异显著 (P <0 .0 5 )。A组大鼠平均存活 (2 4.0± 10 .8)d ,最长 45d ,B组大鼠平均存活 (10 .8± 4.8)d ,最长 2 1d ,两组大鼠生存时间差异非常显著 (P <0 .0 1)。结论 CTLA4Ig基因可以在受体大鼠胰岛细胞或肌肉组织表达 。

Objective To study the expression of CTLA4Ig gene and the effect of its product on the prolongation of the survival of pancreatic islet allograft in diabetic rats. Methods The cells of the islets and muscles of the pancreas were transfected with CTLA4Ig cDNA which was packaged with libofeclin vector. The expression of CTLA4Ig gene was observed after the transplantation of pancreatic islets to see the effect on the prolongation of the survival of the allografts and of the diabetic rats. Results The expression of CTLA4Ig in serum was positive in 2 out of 10 rats in group A with concentrations of 14 ng/ml and 31ng/ml respectively 7 days after the transplantation. The average duration of normal blood glycose level after the transplantation was 14.8±12.3 days in group A and 3.6±5.1 days in group B( P < 0.05). The average survival time of the transplanted rats was 24.0±10.8 days (maximum of 45 days) in group A and 10.8±4.8 days(maximum of 21 days) in group B ( P < 0.01). Conclusion CTLA4Ig gene was expressed in the cells of islets and muscles of the pancreas in the recipient rats and prolonged the survival of the pancreatic islet allograft as well as the recipient rats significantly.