IL-6及其与其他细胞因子联合应用对白血病细胞体外增殖的影响

Effects of interleukin-6 and its combination with other cytokines on the primary leukemic cells in vitro

目的 观察IL 6对急性白血病原代细胞体外生长的作用。方法 应用MTT、CFU L及间接免疫荧光法以IL 6及其与其他细胞因子联合应用对 18例急性白血病患者骨髓细胞的作用进行观察。结果 IL 6体外单独应用对急性白血病原代细胞无明显促增殖及诱导分化作用 ,但与G CSF联合应用则常可抑制原代白血病细胞增殖。结论 IL 6与G CSF联合用于化疗。

Objective To observe the effects of recombinant interleukin 6 (rIL 6) and its combination with other factors on the growth of primary leukemic cells in vitro. Methods The effects of rIL 6 and its combination with G CSF on the growth of primary leukemic cells collected from 18 cases of acute leukemia were observed with MTT, CFU L and indirect immunoflorescent technique. Results rIL 6 exerted no obvious effects on the proliferation or differentiation of the primary leukemic cells in vitro but the combination of rIL 6 and G CSF was able to inhibit those leukemic cells. Conclusion The co administration of IL 6 and G CSF might be more safe to inhibit bone marrow after the patients with acute leukemia are treated with chemotherapy and radiotherapy.