P27蛋白在骨肉瘤中表达的意义及其与预后的关系

Expression of P27 protein in osteosarcoma and its prognostic significance

目的 探讨P2 7蛋白表达与骨肉瘤临床病理、肿瘤细胞增殖活性及其与预后的关系。方法 应用S P免疫组化法检测 80例骨肉瘤P2 7蛋白及增殖细胞核抗原 (PCNA)的表达 ,计算其标记指数 (Labelingindex ,LI)。结果 p2 7LI在 0 %～ 97.5 %之间 ,PCNALI在 9%～ 98%之间 ,而在正常骨及良性骨瘤中不见表达。P2 7蛋白表达与骨肉瘤Dahlin分型、Price分级无关 ,而与WHO分型、PCNALI密切相关。生存率分析显示 ,p2 7LI <48.4%的骨肉瘤预后较好 ,LI >48.4%的骨肉瘤预后较差。结论 骨肉瘤P2 7蛋白表达与肿瘤细胞增殖活性及生存率密切相关 。

Objective To investigate the P27 protein expression in osteosarcoma and its relation to clinicopathological features, tumor cells proliferation and the prognosis. Methods The expression of proliferating cell nuclear antigen (PCNA) and P27 protein was semiquantitatively analyzed with S P immunohistochemistry in a consecutive prospective series of 80 cases of osteosarcoma. Meanwhile, the labeling index (LI) was calculated. Results In all the 80 cases, the range of P27 LI was 0%～97.5% and that of PCNA LI 9%～98%. However, the benign bone tumor normal bone tissue were negative in the staining. The expression of P27 protein was not correlated to Dahlin's type and Price's grade but closely correlated with WHO classification as well as PCNA LI. Furthermore, the patients with a P27 LI less than 48.4% showed a better survival rate than those with a P27 LI more than 48.4%. Conclusion P27 protein expression is closely related to survival in osteosarcoma and PCNA, suggesting that P27 protein may serve as a valuable marker for diagnosis and an indicator to predict the prognosis of osteosarcoma.