血管紧张素Ⅱ对血管外膜成纤维细胞Ⅰ型胶原合成及其mRNA表达的影响

Role of angiotensin Ⅱ on type Ⅰcollagen synthesis and its mRNA expression in vascular adventitial fibroblasts

目的：观察血管紧张素Ⅱ对血管外膜成纤维细胞Ⅰ型胶原合成及其ｍＲＮＡ表达的影响。方法：血管紧张素Ⅱ作用于培养的大鼠血管外膜成纤维细胞，采取酶联免疫吸附法（ＥＬＩＳＡ）、竞争性逆转录聚合酶链反应（ＲＴ－ ＰＣＲ）法分别检测Ⅰ型胶原蛋白合成及Ⅰ型胶原、ｍＲＮＡ的表达。结果：在血管紧张素Ⅱ作用下，血管外膜成纤维细胞Ⅰ型胶原蛋白合成及Ⅰ型胶原ｍＲＮＡ的表达均增加，且有一定的剂量依赖性。结论：血管紧张素Ⅱ可刺激血管外膜成纤维细胞Ⅰ型胶原蛋白合成及Ⅰ型胶原ｍＲＮＡ的表达，提示血管紧张素Ⅱ是调节血管外膜成纤维细胞胶原代谢的重要因子，血管外膜成纤维细胞可能参与高血压血管重塑过程。

AIM:Effects of anatotensin Ⅱ on type Ⅰ collagen synthesis and its mRNA expansion in cultured vascular adventitial fibroblasts. METHODS: Vascular adventitial fibroblasts (VAF)were isolated, cultured from rat thoracic aorta by explant method. ELISA was used to study type Ⅰ collagen synthesis and competihve RT - PCR was employed to detect its mRNA expression after angiotensin Ⅱ administration. RESULTS: Angiontensin Ⅱ caused a dose dependent increase of type Ⅰ collagen synthesis and its mRN expression in VAF. CONCLUSION: The results support that angiotensin Ⅱ is an important factor controlling collagen metabolism of VAF and VAF may play an im- portant role in vascular remodelling of hypertension.