急性肺损伤大鼠多核白细胞与肺组织β受体变化的相关性研究

Relativity of beta adrenoceoptor changes between polymorphonuclear leukocytes and lung tissue in acute lung injury rats

目的：观察急性肺损伤（ＡＬＩ）大鼠循环血多核白细胞（ＰＭＮ）和肺组织β肾上腺素能受体（β－ＡＲ）的变化，并探讨二者β－ＡＲ变化的相关性。方法：静往内毒素复制大鼠ＡＬＩ模型，循环血ＰＭＮ及肺组织β－ＡＲ用放射性配基结合分析法测定。结果：静往内毒素后１、４、６ｈ，大鼠循环血ＰＭＮ及肺组织β－ＡＲ的最大结合容量（Ｂｍａｘ）均明显低于对照组（Ｐ＜０．０１）；静注内毒素后４ ｈ，６ ｈ较１ｈ及６ｈ较４ ｈ ＰＭＮ β－ＡＲ的Ｂｍａｘ均显著降低（Ｐ＜０．０５），肺组织β－ＡＲ Ｂｍａｘ各内毒素组间差异不明显（Ｐ＞０．０５）；循环血ＰＭＮ和肺组织β－ＡＲ变化在程度上无相关性（Ｐ＞０．０５）。结论：ＡＬＩ时循环血ＰＭＮ及肺组织β－ＡＲ数目均明显减少，其在ＡＬＩ发生发展中可能起一定的作用，但ＡＬＩ时外周血ＰＭＮβ－ＡＲ不能作为观察肺组织β－ＡＲ变化的相关指标。

AIM: To observe changes of beta - adrenoceptor (β-AR) and its relativity to polymorphonulear leukocytes (PMN) and acute lung injury (ALI) in rats. METHODS: ALI modle in rat was established by in- travonous injection of E. Coli endotoxin (ET). β - AR was measured by radioligand binding assay with [3H]-dihy- droalprenlol. RESULTS: (1)Maxmal capacity of β - AR (Bmax) in both lung tissue and PMN decreased significantly at the lst, 4th and 6th hours after injection of or. (2)No relativity was found between β-AR Bmax changes of lung tissue and β-AR Bmax changes of PMN. CONCLUSIONS: (1) Decrease of density of 3-AR in lung tissue and PMN may played a role in the development of ALI. (2) The β-AN of PMN in circular bind can not be used as a relative index of β-AR changes of lung tissue in rats with ALI.