利用微卫星多态性揭示酿酒酵母菌株遗传多样性

Genetic Diversity of Saccharomyces cerevisiae Strains Revealed by Microsatellite Sequence Polymorphism

【目的】利用微卫星标记技术对来自新疆和宁夏的48株酿酒酵母菌株进行区分,并分析其亲缘关系。【方法】选择4个微卫星位点:SCAAT1、SCYOR267c、C11和YPL009c,用其多态性对48个菌株进行分析。【结果】4个微卫星标记在48株菌中共检测到87个等位基因,39种基因型;4个位点多态信息含量为0.8593—0.9115,均为高度多态位点;期望杂合度(heterozygosity expected,He)和观测杂合度(heterozygosity observed,Ho)分别为0.8803—0.9268和0.8333—0.9792。【结论】宁夏和新疆地区酿酒酵母菌株生物多样性丰富。

【Objective】A total of 48 Saccharomyces cerevisiae strains from Xinjiang and Ningxia regions were typed by 4 microsatellite markers,and the genetic relations among the strains were also analyzed.【Method】A total of 48 Saccharomyces cerevisiae strains were discriminated for polymorphism at the 4 selected loci： SCAAT1,SCYOR267c,C11,and YPL009c.【Result】A total of 87 alleles,which generated 39 genotypes,were found.The polymorphism information contents（PIC） of all strains at 4 loci were 0.8593-0.9115,suggesting that the 4 SSR loci were hypervariable.The heterozygosity expected and heterozygosity observed were 0.8803-0.9268 and 0.8333-0.9792,respectively.【Conclusion】The results showed the diversity of Saccharomyces cerevisiae strains in Ningxia and Xinjiang regions.