摘要
【目的】利用微卫星标记技术对来自新疆和宁夏的48株酿酒酵母菌株进行区分,并分析其亲缘关系。【方法】选择4个微卫星位点:SCAAT1、SCYOR267c、C11和YPL009c,用其多态性对48个菌株进行分析。【结果】4个微卫星标记在48株菌中共检测到87个等位基因,39种基因型;4个位点多态信息含量为0.8593—0.9115,均为高度多态位点;期望杂合度(heterozygosity expected,He)和观测杂合度(heterozygosity observed,Ho)分别为0.8803—0.9268和0.8333—0.9792。【结论】宁夏和新疆地区酿酒酵母菌株生物多样性丰富。
【Objective】A total of 48 Saccharomyces cerevisiae strains from Xinjiang and Ningxia regions were typed by 4 microsatellite markers,and the genetic relations among the strains were also analyzed.【Method】A total of 48 Saccharomyces cerevisiae strains were discriminated for polymorphism at the 4 selected loci: SCAAT1,SCYOR267c,C11,and YPL009c.【Result】A total of 87 alleles,which generated 39 genotypes,were found.The polymorphism information contents(PIC) of all strains at 4 loci were 0.8593-0.9115,suggesting that the 4 SSR loci were hypervariable.The heterozygosity expected and heterozygosity observed were 0.8803-0.9268 and 0.8333-0.9792,respectively.【Conclusion】The results showed the diversity of Saccharomyces cerevisiae strains in Ningxia and Xinjiang regions.
出处
《中国农业科学》
CAS
CSCD
北大核心
2012年第12期2537-2543,共7页
Scientia Agricultura Sinica
基金
国家葡萄产业技术体系建设专项经费资助(CARS-30-jg-03)
关键词
酿酒酵母
微卫星标记
菌株区分
生物多样性
Saccharomyces cerevisiae microsatellite markers strain typing biodiversity