三个高花药培养力小麦材料培养力性状的配合力分析

Combining Ability Analysis on Anther Culture Traits of Three Wheat Genotypes with High Regeneration Ability

为了给小麦花培育种提供参考,以3个高花药培养力的小麦材料为母本、4个农艺性状较好的小麦品种为父本,按不完全双列杂交方法配制了12个组合,对12个组合F1的愈伤组织诱导率、绿苗分化率和绿苗产率3个性状进行了检测,并进行了亲本及组合的一般配合力和特殊配合力效应分析。结果表明,3个高花药培养力小麦材料的愈伤组织诱导率、绿苗分化率和绿苗产率3个性状一般配合力效应的表现不尽相同。3个高培养力小麦材料中H6123和H60148的绿苗产率一般配合力表现正效应,其中H6123绿苗产率的一般配合力相对效应值最高,达到18.61,是一个优良的花培亲本。12个组合中有7个组合的绿苗产率特殊配合力表现正效应,且其中的5个组合中都含有1个绿苗产率一般配合力相对效应值较高的亲本,说明高配合力的亲本材料在组合配制中具有重要的作用。

Analysis on the combining ability of the anther culture traits was conducted with 3 wheat genotypes of H60148,HLM2 and H6123,which with high regeneration ability via anther culture.A set of 3×4 incomplete diallel crosses were made between the above 3 genotypes and 4 high yielding cultivars of Xiaoyan 22,Jimai22,Aikang58 and Dekang961.The results showed that genotype significantly affected anther culture ability in terms of callus induction frequency,green plantlet regeneration frequency and green plantlet yield.The effects of general combining ability（GCA） and specific combining ability（SCA） were significant for callus induction frequency,green plantlet regeneration frequency and green plantlet yield.GCA/SCA ratio for callus induction frequency,green plantlet regeneration frequency and green plantlet yield was higher than 1.0,indicating the importance of additive genetic variation in these materials.H6123 and H60148 showed the positive GCA values for green plantlet yield.H6123 has the highest GCA effect value among the three female parent genotypes and could be a good inter-parent for wheat breeding via anther culture.Among the 12 crosses,there were seven crosses showing positive SCA effect value for green plantlet yield.The use of parent with high general combining ability and high anther culture ability could lead to the efficient production of green plantlets from the F1 crosses via anther culture.