不同生育期转双价(Bt+CpTI)基因抗虫棉根际土壤酶活性和养分含量变化

Changes of enzyme activities and available nutrients in rhizosphere soil of transgenic Bt+CpTI cotton during its growth period

田间试验条件下,以转双价(Bt+CpTI)基因棉SGK321及其非转基因亲本常规棉石远321为研究对象,比较分析不同生育期(30、60、90、120d)转双价基因棉和亲本棉根际土壤酶活性(脲酶、碱性磷酸酶、过氧化氢酶)及速效养分(硝态氮、铵态氮和速效磷)的变化。结果表明,转双价基因棉根际土壤硝态氮、铵态氮和速效磷含量变化趋势与其亲本常规棉基本一致,但各养分的具体变化幅度因生育期不同而异。播种后30、60、120d转双价基因棉和亲本常规棉根际土壤碱性磷酸酶活性无显著差异;土壤脲酶和过氧化氢酶活性随棉花生育进程其变化趋势虽各不相同,但同一生育期转双价基因棉与亲本常规棉根际土壤脲酶和过氧化氢酶活性均无显著差异。表明,土壤酶活性和速效养分含量受转双价基因棉的影响较小,其变化主要受生育期的影响。

This paper studied the changes of urease,alkaline phosphatase and catalase activities and nitrate nitrogen,ammonium nitrogen,and available phosphorus contents in the rhizosphere soils of transgenic Bt＋CpTI cotton and its parental isoline in different growth periods（30,60,90,and 120 d after sowing） under field condition.There was a similar change trend in the rhizosphere soil nitrate nitrogen,ammonium nitrogen,and available phosphorus contents between transgenic Bt＋CpTI cotton and its parental isoline,but the change range varied with growth period.The rhizosphere soil alkaline phosphatase activity had less difference between transgenic Bt＋CpTI cotton and its parental isoline 30,60,and 120 d after sowing.Though the urease and catalase activities in the rhizosphere soils had different change trend with the growth of transgenic cotton and its parental isoline,no significant difference was observed in the two enzyme activities at the same growth periods between the two cotton cultivars.Our results indicated that the enzyme activities and available nutrient contents of rhizosphere soil were less affected by transgenic cotton,but mainly affected by the growth period.