酰胺酶催化底物特异性的研究进展

Advance in the Substrate Specificity of Amidase

酰胺酶是一类催化酰胺水解成相应羧酸的水解酶,其作用底物谱广,有报道的酰胺酶可以广泛作用于脂肪、芳香、杂环氨基酰胺类酰胺,但是酶活差别巨大。当底物为芳香、杂环类酰胺尤其是存在邻位取代基时,酰胺酶的活力受到显著干扰,这种干扰与空间位阻和电子效应有关。

Amidases were a group of enzymes that can biocatalyze amides to the corresponding acids. Although different amidases had different substrate specificity, most of them can act on a wide range of amides including aliphatic, aromatic, hetercyclic and amino amides. However, the activity differed in thousands of ways. Their activity was critically influenced when the enzymes acted on the aromatic and hetercyclic substrates, especially that those with ortho-substituents, which was related to steric hindrance and electronic effect.