摘要
[目的]预测二斑叶螨对螺螨酯(240 g/L SC)产生抗药性的速度,指导合理用药。[方法]以选择压力为杀死种群50%～60%的剂量,对二斑叶螨敏感种群进行室内汰选,每隔5代用浸叶碟法和叶片残毒法对种群进行测定,求得毒力回归方程。[结果]经过25代汰选,二斑叶螨的卵对螺螨酯抗性指数仅上升至1.28倍,并未产生明显抗性。汰选至35代,对幼螨的LC50值(制剂量)由15.1252 mg/L升高至127.2624 mg/L,获得抗性指数8.41倍的抗性种群。[结论]在室内汰选35代后,二斑叶螨的幼螨虫态对240 g/L螺螨酯SC已产生明显抗药性。
[Aims] The resistance velocity of Tetranychus urticae Koch （T. urticae） to spirodiclofen 240g/L SC was monitored to direct to use acaricides in a resonable way. [Methods] Selected a susceptible strain of T. urticae under pressure of killing about 50-60% population with spirodiclofen 240 g/L SC. The population was tested every five generations by using both leaf disk dip and leaf residue methods, and LC-P line was obtained. [Results] After 25 selection cycles with spirodiclofen 240 g/L SC, resistance ratio of T. urticae eggs only rose to 1.28 times. The egg stage remained far more susceptible to spirodiclofen 240 g/L SC. On the other hand, LC50 value of larvaes increased from 15.1252 to 127.2624 mg/L when up to the 35th selection cycles Obtaining a strain with a resistance ratio of 8.41, determined on larval stage of T. urticae. [Conclusions] After the 35th selection cycles, more obvious resistance of larvaes has already formed.
出处
《农药》
CAS
北大核心
2012年第7期529-530,535,共3页
Agrochemicals
基金
辽宁省教育厅项目(2009S094)
关键词
二斑叶螨
螺螨酯
汰选
抗药性
Tetranychus urticae
spirodiclofen
selection
resistance
