利用cDNA-AFLP研究辣椒细胞核雄性不育差异表达基因

Differentially Distinguished Expressed Genes in Chili Pepper Genic Male Sterility (GMS) Using cDNA-AFLP

辣椒细胞核雄性不育两用系在辣椒杂交种配制方面有着非常重要的应用,为了进一步了解其不育性的分子机理,利用cDNA-AFLP对辣椒细胞核雄性不育两用系‘AB23’可育株和不育株花蕾的mRNA指纹图谱进行了初步分析。结果表明:共获得19个差异表达的TDF,均来自可育株。在可育株花蕾中得到了19个TDFs,其中13个TDFs功能已知,2个功能未知,4个没有找到同源序列。对13个已知序列的分析表明,这些基因涉及了细胞的代谢、转录、胞间运输和信号转导等相关过程。通过半定量RT-PCR技术对获得的19个TDFs进行验证,其表达特征与cDNA-AFLP结果一致。

Genic male sterility is an important way to utilize the heterosis of chili pepper,So,pilot studies were conducted for better understanding the mechanism of the chili pepper GMS,establish chili fertile plants buds and sterility plant buds cDNA library between the homozygous genic male sterility two-type line ‘AB23’ and by using cDNA-AFLP in chili pepper genic male sterility of sterility genes for preliminary research.The results showed that there were 19bands expressed in a higher level in fertile plant buds,Among them,13bands homologous sequences function known,2bands function unknown and 4bands were maybe some novel genes.The 13 homologous sequences function known participated in the following biological process ： metabolism,transcription,cellular transport,signal transduction mechanism etc.Furthermore,RT-PCR demonstrates that it was consistent with the result of cDNA-AFLP.This study result will help us understand that the development of chili pepper male gametes and could give basic information about the genic male sterility in chili pepper.