桑菊清解汤对大鼠呼吸机相关性肺损伤的影响

Effects of Sangju Qingjie Decoction on ventilator-induced lung injury in rats

目的评价桑菊清解汤对大鼠呼吸机相关性肺损伤的影响。方法健康成年SD大鼠36只，雌雄不拘，体重300～350g，采用随机数字表法，将其随机分为3组（Ⅱ=12）：对照组（C组）、机械通气组（V组）和桑菊清解汤组（SJ组）。采用大潮气量（VT=40ml／kg）机械通气2．5h制备大鼠呼吸机相关性肺损伤模型。SJ组于术前10d采用桑菊清解汤300g灌胃，1次／d，于第10次灌胃后2h时开始制备模型，V组和C组给予等容量生理盐水。于机械通气前（Tn）、机械通气结束（T1）、机械通气后30min（T1）时采集股动脉血样测定动脉血气，计算呼吸指数（RI）和氧合指数（01），然后处死大鼠，取肺组织，采用双抗体夹心ELISA法测定肺组织TNF—α、IL-6和IL-10的含量，测定肺组织湿／干重（W／D）比，光镜下观察肺组织病理学结果。结果与C组比较，V组和SJ组T1,2时RI升高，OI降低，肺组织TNF-α、IL-6、IL-10含量和W／D比升高（P〈0．05）；与V组比较，SJ组RI降低，OI升高（P〈0．05），SJ组肺组织TNF—α、IL-6含量和W／D比降低，IL-10含量升高（P〈0．05）。SJ组肺组织病理学损伤较v组减轻。结论桑菊清解汤可减轻呼吸机相关性肺损伤，其机制与抑制炎性反应有关。

Objective To investigate the effects of Sangju Qingjie Decoction on the ventilator-induced lung injury in rats.Methods Thirty-six healthy male SD rats of both sexes, weighing 300-350 g, were randomly divided into 3 groups （ n = 12 each） ： control group （group C） ; mechanical ventilation group （group V） and Sangju Qingjie Decoction group （group SJ）. The animals were anesthetized with intraperitoneal 3% urethane 1.4 g/kg and tracheostomized. The femoral artery and vein were eannulated for blood sampling and drug administration. The animals were mechanically ventilated for 2.5 h （VT = 40 ml/kg, RR 40 bpm, I ： E = 1 ： 1, FiO2 21% ）. In group SJ, Sangju Qingjie Decoction 300 g was injected into the stomach through a gastric tube once a day for 10 consecutive days, and mechanical ventilation was performed 2 h after the last injection. While in groups V and C, the equal volume of normal saline was given instead of Sangju Qingjie Decoction. Arterial blood samples were collected before ventilation, at the end of ventilation, and at 30 min after ventilation （ T0-2 ） for blood gas analysis. Respiratory index （RI） and oxygenation index （OI） were calculated. The animals were then sacrificed and the lungs were removed for microscopic examination and determination of the content of TNF-α, IL-6 and IL-10 and W/D lung weight ratio. Results Compared with group C, RI at T1,2 , the content of TNF-α, IL-6 and IL-10 and W/D ratio were significantly increased, and OI was significantly decreased at T1,2 in groups V and SJ （P 〈 0.05） . Compared with group V, RI at T1,2, the content of TNF-a and IL-6 and W/D ratio were significantly decreased, and OI at T1,2 and the content of IL-10 were significantly increased in group SJ （P 〈 0.05）. The pathological damage was attenuated in group SJ compared with group V. Conclusion Sangju Qingjie Decoction can attenuate the ventilator-induced lung injury through inhibition of inflammatory response in rats.