陆海杂交棉叶绿素荧光参数及生理动态变化研究

Study on the Effect of Interspecific Hybridization Island Cotton Varities on the Chloro Phyll Fluoreseence Parameters and Physiological Biochemistry Dynamic Change

目的:研究陆海杂交棉新陆中31号叶绿素荧光参数及生理动态变化规律,为陆海杂交棉栽培管理、高产机理研究提供参考。方法:以新陆中31号、新海21号、中棉49号为试验材料,在棉花整个生育期,研究叶绿素荧光参数、可溶性糖、可溶性蛋白以及POD活性和CAT活性的变化。结果:在整个生育期,参试材料的Fv/Fm、Fv/Fo、qP、Yield和ETR、可溶性糖含量、可溶性蛋白含量和POD酶和CAT酶活性基本上呈现出相同的变化趋势,即随着生育期变化呈现出先增高后降低的趋势,总体上表现为盛花期最高,之后逐渐开始降低,而qN值的变化则不同。另外,在相同条件下,新陆中31号的Fv/Fm,Fv/Fo、qP、可溶性糖含量、可溶性蛋白含量、POD酶和CAT活性总是高于两个常规棉对照品种,说明在同等栽培管理条件下,该品种清除活性氧的能力较高,属于典型的高光效品种。结论:在相同栽培条件下,叶绿素荧光参数、可溶性糖含量、可溶性蛋白含量、POD酶和CAT酶活性的变化与基因型之间存在着密切相关,其中陆海杂交棉新陆中31号清除活性氧的能力较高。

Objective： The rule of chorophyll fluorescence parameters and physiological dynamical change of interspecific hybridization island cotton varities xinluzhongNo. 31 and to provide a reference of cultivation and mechanism of high yield. Method：ZhongmianNo. 49 and Xinhai No. 21 were analysised and compared differences of several indicators of chlorophyll fluoresceence parameters, soluble carbohydrate contents, soluble protein contents, POD and CAT activities in the cotton entire growth period. Result：The results showed that the indicators of Fv/Fm, Fv/Fo,qP,Yield, ETR, soluble carbohydrate content, soluble protein content, POD and CAT activities basically had high-low trends with growth period change besides of qN value, and the curve was the highest value at the full blooming stare and then gradually decreased. But under the same condition,these values of Fv/Fm,Fv/Fo,qP, Yield,ETR,soluble carbohydrate content, soluble protein content, POD and CAT activities on condy 4036 always were higher than other two check cotton varieties. So under the same level of cultivation management condition, this variety was a tipically high-efficiency light and had very high ability of eliminating reactive oxygen. Conclusion： Under the same condition, Fv/Fm, Fv/Fo, qP, Yield, ETR, soluble carbohydrate content, soluble protein content, POD and CAT activities of these experimental material were highly correlated with genotypes,but Interspecific Hybri dization island cotton varieties had a higher ability of scavenging reactive oxygen than the compare varieties.