芒柄花素对PDGF-BB所致血管平滑肌细胞迁移和增殖的影响

Formononetin inhibits Vascular Smooth Muscle Cells migration and proliferation induced by PDGF-BB

目的研究芒柄花素(Formononetin,Formo)对血小板源性生长因子BB型(PDGF-BB)所致血管平滑肌细胞(VSMC)增殖和迁移的影响并探讨其可能的机制。方法组织块贴壁法培养SD大鼠胸主动脉平滑肌细胞,结晶紫染色法观察芒柄花素(0.3、1、3、10μM)对PDGF—BB(40ng/ml)所致的VSMC增殖的影响;BrdU摄取法考察芒柄花素对PDGF—BB所致DNA合成率的影响;划痕实验研究芒柄花素对PDGF—BB所致细胞迁移活力的影响;免疫沉淀法检测芒柄花素对PDGFR磷酸化水平的影响。结果芒柄花素在1-10μM浓度范围内呈剂量依赖性抑制PDGF-BB诱导的VSMC增殖和DNA合成(p<0.05),芒柄花素在3、10μM浓度下抑制PDGF-BB诱导的VSMC迁移(p<0.05)。芒柄花素抑制PDGF-BB对PDGFR受体磷酸化的激活作用。结论芒柄花素抑制PDGF-BB诱导的VSMC迁移和增殖,其作用机制可能与其抑制平滑肌细胞上PDGFR受体磷酸化有关。

Objective To investigate the effect of formononetin on the proliferation and the migrating activity of vascular smooth muscle cells（VSMCs） induced by Platelet-derived growth factor BB （PDGF-BB）. Methods The effect of formononetin on the proliferation of VSMCs induced by PDGF-BB.was investigated by using crystal staining and BrdU incorporation assay. The effect of formononetin on the migrating activity of VSMCs induced by PDGF-BB by using wound healing assay. Western Blotting was used to detect the phosphorylation level of PDGFR. Results Formononetin inhibits PDGF-BB induced VSMC proliferation and migration in the concentration range from 0.3 to 10 μM. The inhibition of SalA on VSMC proliferation and migration is associated with the inhibition of phosphorylation of PDGFR. Conclusion Our results suggest that Formononetin inhibits migration and proliferation of VSMCs induced by PDGF-BB via the inhibition of the PDGFRβ phosphorylation.