芘高效降解菌ZQ_5的培养条件及无机元素对其降解效率的影响

Influence of cultural conditions and inorganic elements on degradation of pyrene-degrading strain ZQ_5

以从我国最大的石油污水灌区之一——沈抚灌区污染土壤分离到的以芘为惟一碳源、能源生长的高效降解菌株ZQ5为实验材料,通过对菌株ZQ5培养条件的优化,以及采用摇瓶振荡培养方法测定菌株ZQ5对不同浓度芘的降解率,表明:菌株ZQ5在30℃振荡培养16 d后对150 mg/L芘的降解率为90.31%。通过模拟稻田施用N、P和K肥等的土壤环境,探索了无机营养元素对降解菌ZQ5降解能力的影响,发现土壤中混合加入N、P和K无机营养元素的降解率能达到82%以上,比单加某种营养元素对降解菌ZQ5的降解效果好。本研究结果可以指导稻田PAHs的原位生物修复。

The pyrene-degrading strain ZQ5,which was isolated by selective enrichment culture from oil- contaminated soils of Shenfu irrigation area, was able to grow on pyrene as a sole carbon and energy source. Used as the based test material, strain ZQ5 ＇ s culture condition was optimized. After shaking flask culture, the degradation rate of pyrene for strains ZQ5 was determined at different pyrene concentrations. The results showed that the degradation rate of pyrene was 90.31% at the initial pyrene concentration of 150 mg/L and 300C for 16 days. Imitating paddy environment through adding N, P, K fertilizer was used to research the influence of inor- ganic nutrient elements on the strain ZQs degradation capacity. The result indicated that mixed inorganic nutrient elements could better promote degradation compared to a single inorganic nutrient elements to the strain ZQ5, and the degradation efficiency reached 82%. So the research can be widely applied to situ bioremediation of paddy contaminated bv PAHs.