人羊膜对大鼠皮肤切创愈合中MMP-9表达影响的实验研究

Experimental study on the effect of human amnion on MMP-9 expression during healing process of rat skin incised wound

目的:研究人羊膜对大鼠皮肤切创愈合过程中感染率的影响及基质金属蛋白酶-9(Matrix metalloproteinase-9,MMP-9)表达影响的研究及意义,探讨其促进皮肤愈合的机制,为其在临床的应用提供实验依据。方法:建立大鼠皮肤切创模型,观察切口愈合时间、感染率;在各时相点处死取材,应用免疫组织化学技术,通过计算机图像分析系统检测人胎羊膜对不同损伤时间大鼠皮肤组织中MMP-9的表达。结果:新鲜羊膜组感染率(6.7%)与对照组(33.3%)比较,创面感染率明显低于对照组,差异有统计学意义(P<0.05);在伤后12 h、1天、2天、3天,新鲜羊膜组MMP-9的表达高于对照组,差异有统计学意义(P<0.05),5天、7天两组的表达差异无统计学意义(P>0.05)。结论:羊膜贴附创口能减少创口的感染率;在大鼠皮肤切创愈合的早期阶段,可通过促进创缘周围MMP-9的表达,加速清创,促进创口的愈合。

Objective： To research the effect of human amnion on infection rate and matrix metalloproteinase -9 （MMP -9） expression during healing process of rat skin incised wound and its significance, explore the mechanism of its promoting effect on healing of rat skin, provide an experimental basis for the clinical application. Methods： The models of rat skin incised wound were established, the wound healing time and infection rate were observed; the rats were killed at different time points; immunohistochemical method was used to detect the effect of human amnion on MMP -9 expression in skin tissue at different time points by computer image analysis system. Results： The wound infection rate in fresh amnion group was 6. 7%, which was significantly lower than that in control group （33.3%） （P 〈 0. 05 ） . At 12 hours, one, two, and three days after establishing models, the expression levels of MMP -9 in fresh amnion group were significantly higher than those in control group （ P 〈 0.05 ）, but there was no statistically significant difference in the expression levels of MMP - 9 at 5 and 7 days after establishing models between the two groups （P 〉0. 05） . Conclusion： Amnion adhered to wound can reduce the infection rate of incised wound ; at early stage of rat skin incised wound healing, amnion can promote wound debridement and healing by promoting the expression of MMP -9 around the wound edge.