固定化脂肪酶性能研究及在洗毛中的应用

Stability of immobilized lipase and its application on wool scouring

利用硅藻土吸附法对解酯假丝酵母发酵产生的脂肪酶进行了固定化,研究了固定化酶和游离酶的酶学性质,以及用固定化脂肪酶对羊毛进行生物酶法洗毛。结果表明:固定化酶比游离酶的最适作用温度提高了5℃,最适作用pH值向碱性方向移动了0.5个单位,热稳定性和pH值稳定性有了大幅度提高。在固定化酶的最适作用pH值下,得到最佳的洗毛条件为:温度45℃、浴比1∶35、时间20 h。

We entrap lipase from Candida lipolytica by diatomite adsorption,and study the enzymatic properties of immobilized enzyme and free enzyme, we also use immobilized lipase for enzymatic wool scouring. The results show that the optimal temperature of the immobilized enzyme is increased 5 ℃ than free enzyme, and its optimum pH value mobiles to the alkaline direction by 0. 5 units, with greatly improved thermal and pH value stability. Under the optimal reaction pH value of the immobilized enzyme, the optimal parameter of the variables are determined as：temperature 45 ℃ ,bath ratio 1：35 and time 20 h.