响应面法优化水溶性大豆多糖-铁(Ⅱ)配合的合成工艺

Optimization of Synthesis of Soluble Soybean Polysaccharide-Iron (Ⅱ) Complex by Response Surface Methodology

优化水溶性大豆多糖-铁(Ⅱ)配合物的合成工艺,在单因素试验基础上,应用响应面法二次回归正交旋转组合试验设计,分析水溶性大豆多糖与催化剂柠檬酸三钠的质量比、pH值、反应时间及温度对配合物铁含量的影响,建立相应的预测模型。方差分析结果表明:质量比、pH值对铁含量有显著影响。优化所得的较优工艺参数为水溶性大豆多糖与柠檬酸三钠质量比1.89:1、pH3.89、反应时间1.56h、温度60.6℃。对应的铁含量的预测值为23.08%,实际平均值为21.89%。结果表明:应用响应面法所得到的水溶性大豆多糖-铁(Ⅱ)配合物的合成工艺参数是可行的。

In order to optimize the synthesis process of soluble soybean polysaccharide-iron （ II ） complex, based on the single factor tests, a dual quadratic rotary combination design was used to analyze the effects of mass ratio between soluble soybean polysaccharide and catalyst sodium citrate, pH, reaction time and reaction temperature on iron content of SSPS-Fe （ II ）. A prediction model was established. The analysis of variance revealed that mass ratio and pH could significantly affect iron content of SSPS-Fe （ II ）. The optimal synthesis conditions were mass ratio between soluble soybean polysaccharide and catalyst sodium citrate of 1.89：1, pH 3.89, reaction time of 1.56 h and reaction temperature of 60.6℃. The iron content predicted by the model was 23.08%, which was consistent with the experimental value.