GPC-HPLC-FLD法测定动植物油脂中的苯并(a)芘

Determination of Benzo(a) pyrene in Animal Fats and Vegetable Oils by Gel Permeation- High Performance Liquid Chromatography with Fluorescence Detector

建立动植物油脂中苯并(a)芘残留量的凝胶净化-高效液相色谱-荧光检测的测定方法。方法采用凝胶色谱净化系统处理样品,可除去油脂中甘油三酯等杂质的干扰。色谱条件:Hypersil ODS2(150mm×4.6mm,5μm)反相色谱柱,流动相为甲醇-水(94:6,V/V),流速1.0mL/min,激发波长365nm,发射波长406nm,柱温30℃。方法的检出限0.1μg/kg,线性范围0.1～50μg/kg,加标回收率92.0%～106.0%,相对标准偏差为1.86%(n=6)。该法具有样品预处理简单、灵敏度高、精密度高等优点,可用于动植物油脂样品中苯并(a)芘的快速准确测定。

This paper describes a method to determine benzo（a） pyrene in animal fats and vegetable and oils using gel permeationhigh performance liquid chromatography with fluorescence detector（HPLC-FLD）.In this method,gel permeation cleanup system was used to remove the interference such as triglyceride.Chromatographic separation was achieved by a reverse-phase column of Hypersil ODS2 with the mobile phase consisting of methanol-water（94：6,V/V） at a flow rate of 1.0 mL/min.FLD were set at 365 nm excitation and 406 nm emission wavelengths,and the column temperature was kept at 30 ℃.The limit of detection was 0.1μg/kg,the calibration curve was linear between a concentration range of 0.1-50μg/kg,the recoveries were in the range of 92.0%-106.0%,and RSD（n = 6） value was 1.86%.The method is simple,rapid,accurate and sensitive for directly determining benzo（a）pyrene in animal fats and vegetable oils without complicated and time-consuming separation process.