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山柰酚对成骨细胞株MG-63增殖分化的影响 被引量:12

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摘要 目的探讨山柰酚对成骨细胞增殖分化的影响。方法将含有ERs配体结合域(LBD)的质粒pCMV-BD和pFR-Luci报告基因及内标质粒beta-gal共转染到MG-63成骨细胞中,检测山柰酚与雌激素受体的关系;Celltiter试剂检测山柰酚对细胞活力的影响,茜红素染色和pNPP法检测山柰酚对成骨细胞分化和矿化能力的影响,流式细胞仪检测山柰酚对细胞内游离钙的影响。结果山柰酚能够激活雌激素受体的活性,促进成骨细胞增殖,提高成骨细胞分化和矿化能力。结论山柰酚能够促进成骨细胞的增殖和矿化能力。
出处 《时珍国医国药》 CAS CSCD 北大核心 2012年第6期1463-1465,共3页 Lishizhen Medicine and Materia Medica Research
基金 广东省科技厅基金(No.2007B031408004)
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