黄精对2型糖尿病胰岛素抵抗大鼠葡萄糖转运蛋白-4基因表达的影响

Effects of rhizoma polygonati on the expression of glucose transporter-4 gene in type 2 diabetes mellitus rats with insulin resistance

目的观察黄精水提液对2型糖尿病(T2DM)胰岛素抵抗大鼠肌肉组织葡萄糖转运蛋白-4(GLUT-4)基因表达的影响。方法采用小剂量链脲佐菌素加高脂高热量饲料喂养方法建立2型糖尿病胰岛素抵抗模型,将造模大鼠随机分为模型对照组和黄精高、中、低剂量治疗组(分别灌胃10.0、5.0、2.5 g·kg-1·d-1),另选10只为正常对照组。灌胃8周后,空腹取材,反转录-聚合酶链反应法检测肌肉组织GLUT-4基因mRNA水平。结果与正常对照组相比,模型对照组及黄精高、中、低剂量治疗组大鼠GLUT-4基因mRNA表达减少,空腹血糖(FPG)显著升高,差异均有统计学意义(P<0.01)。与模型对照组比较,黄精高、中、低剂量治疗组FPG均降低,其中以中、高剂量治疗组降低显著,差异有统计学意义(P<0.01);黄精高、中、低剂量治疗组GLUT-4基因mRNA表达均增高,其中以中、高剂量治疗组增高显著,差异有统计学意义(P<0.01)。结论黄精水提液具有增强T2DM胰岛素抵抗大鼠GLUT-4基因表达,从而降低血糖的作用。

Objective To observe the effects of rhizoma polygonati（RP） on the expression of glucose transporter-4（GLUT-4） gene in muscular tissue in type 2 diabetes mellitus（T2DM） rats with insulin resistance（IR）.Methods The model of T2DM rats with insulin resistance was established by giving high fat,high caloric diet and injection of small dose of streptozotocin（STZ）.The model rats were divided into model control group,high,middle and low doses RP groups（intragastric administration 10.0,5.0,2.5 g·kg-1·d-1 RP）.Other 10 rats were selected as normal control group.After intragastric administration for eight weeks,then the GLUT-4 mRNA in muscular tissue was determined by reverse transcription-polymerase chain reaction（RT-PCR）.Results Compared with normal control group,the expressions of GLUT-4 mRNA were lower（P〈0.01）,but the levels of fasting plasma glucose（FPG） were higher（P〈0.01） in model control group,low,middle and high doses RP groups.Compared with model control group,the levels of FPG were lower in low,middle and high doses RP groups,and the decreasing level in middle and high doses RP groups was obviously（P〈0.01）;the expressions of GLUT-4 mRNA were higher in low,middle and high doses RP groups,and the increasing level in middle and high doses RP groups was obviously（P〈0.01）.Conclusion RP can reduce blood glucose by increasing expression of GLUT-4 mRNA in T2DM rats with IR.