短程全反式维甲酸对人结肠癌LoVo细胞增殖和凋亡的影响

Effect of short-term all-trans retinoic acid on cell proliferation and apoptosis of human colorectal cancer LoVo cell

目的探讨短程全反式维甲酸(ATRA)对人结肠癌LoVo细胞增殖和凋亡的影响。方法四甲基偶氮唑盐(MTT)法检测ATRA对LoVo细胞的生长抑制率并筛选合适的药物用量,流式细胞仪检测ATRA诱导的肿瘤细胞周期和凋亡率变化。结果选择1.0μmol·L-1ATRA作为进一步实验的工作浓度。1.0μmol·L-1ATRA作用12 h后G1期细胞开始增多,48 h后G1期细胞明显增多并伴S期、G2/M期细胞减少(P<0.05)。1.0μmol·L-1ATRA作用48、72 h组的早期凋亡率和总凋亡率与对照组比较差异均有统计学意义(P<0.01);ATRA作用48、72 h组的早期凋亡率和总凋亡率的组间比较差异均有统计学意义(P<0.05)。结论短程1.0μmol·L-1ATRA主要通过阻滞LoVo细胞于G1期并诱导其细胞发生早期凋亡,可明显抑制肿瘤细胞的增殖。

Objective To investigate the effect of short-term all-trans retinoic acid（ATRA） on cell proliferation and apoptosis of human colorectal cancer LoVo cell.Methods The growth inhibitory rate of ATRA on LoVo cell was detected by 3-（4,5-dimethylthiazol-2-y1）-2,5-diphenyltetrazolium bromide（MTT） and the suitable drug concentration of ATRA was defined.Cell cycle and apoptosis induced by ATRA were assessed by flow cytometry.Results 1.0 μmol·L-1ATRA was chosen as work concentration for further experiment.The cells in G1 phase began to increase after 1.0 μmol·L-1ATRA treatment for 12 hours,and the cells in G1 phase were significance increase,while the cells in S and G2/M phase decreased after treatment for 48 hours（P〈0.05）.Compared with control group,there were statistical significance in early and total apoptosis rates of LoVo cell induced by 1.0 μmol·L-1 ATRA treatment for 48 and 72 hours groups（P〈0.01）;there was statistical significance in early and total apoptosis rates of LoVo cell induced by ATRA between treatment for 48 and 72 hours groups（P〈0.05）.Conclusion A short-term 1.0 μmol·L-1 ATRA can significantly inhibit LoVo cell growth by blocking the cells into G1 phase and inducing the cells early apoptosis.