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不同时间点GABA_A受体α_2亚基在神经病理性痛中的表达 被引量：1

Expression of GABA_A Receptor α_2 Subunit in Neuropathic Pain at Different Time Points
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摘要 为探讨慢性坐骨神经压榨性损伤(CCI)模型背根神经节GABAA受体α2亚基在不同时间点(3、7、12d)表达。采用雄性SD大鼠28只,随机分为4组(n=7)。A组(假手术组)、B组(CCI术后3d)、C组(CCI术后7d)、D组(CCI术后12d),取L4-L6背根神经节做冰冻切片,用免疫荧光染色方法观察GABAA受体α2亚基表达变化。结果显示,(1)CCI模型鼠热缩足反射潜伏期与假手术组相比显著缩短(P<0.01)。(2)免疫荧光染色示A组、B组、C组、D组DRG神经元均有GABAA受体α2亚基表达,分布于DRG各种直径神经元细胞膜上。(3)与A组比较,D组L4-L6背根神经节GABAA受体α2亚基受体的表达减少(P<0.05);B组、C组变化不明显(P>0.05)。由此可知,慢性坐骨神经压榨性损伤(CCI)模型背根神经节GABAA受体α2亚基表达下调,提示GABAA受体α2亚基参与了神经病理性痛的发生。 To investigate the expression of dorsal root ganglia GABAA receptor α2 subunit in sciatic nerve chronic crush injury （CCI） model at different time points （3,7,12 d）. Methods：Twenty-eight male Sprague Dawley rats were randomly divided into 4 groups：group A of sham operation ,group t3 3 days after CCI;group C 7 days after CCI,and group D 12 days after CCI. L4-L6 dorsal root ganglia were used for frozen sections. Immunofluorescence staining was applied to observe the changes in GABAA receptor α2 subunit. Results ： （1） CCI injured side produced significantly thermal with drawal latency shorter （ P〈 0.01 ）. （2） The immunofluorescence staining show A,B,C and D group of DRG neuron nave GABAA receptor α2 subunit expression,located in the DRG each kind of diameter neuron cell membrane. （3）Compared with group A, the expression of L4-L6 dorsal root ganglia GABAA receptor variousaz subunit receptor in group D decreased （P〈0.05） ;no significant changes in group 13 and group C （P 〉0.05）. Conclusion：The decrease in the expression of GABAA receptor α2 subunit in sciatic nerve chronic crush injury （CCI） model of dorsal root ganglia suggests that GABAA receptor α2 subunit has a correlation with neuropathic pain.
作者 连宇 司军强 马克涛 尚媛媛 李丽
机构地区 石河子大学医学院/新疆地方与民族高发病教育部重点实验室
出处 《石河子大学学报（自然科学版）》 CAS 2012年第2期202-205,共4页 Journal of Shihezi University(Natural Science)
基金 新疆兵团青年科技创新基金专项(2010JC33)
关键词 GABAA受体α2亚基 神经病理性痛 背根神经节 免疫荧光 GABAA Rα2 neuropathic pain dorsal root ganglion immunofluorescence
分类号 R338 [医药卫生—人体生理学]
  • 相关文献

参考文献11

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共引文献6

同被引文献15

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石河子大学学报（自然科学版）
2012年 第2期

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