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溶血磷脂酸对小胶质细胞活化和吞噬功能的影响

Effect of LPA on activation and endocytosis of microglia
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摘要 目的:观察溶血磷脂酸(LPA)对小胶质细胞活化及其吞噬功能的影响。方法:小胶质细胞系BV2细胞复苏后传代培养,取对数生长期细胞分为对照组和LPA组,LPA干预30min后各组加入FITC标记的葡聚糖,在干预后1h、2h后收取细胞,用流式细胞仪测定各孔FITC-葡聚糖微粒吞噬率及荧光强度。结果:LPA干预1h时,吞噬率较对照组明显增加(P<0.05),2h时两组细胞吞噬率达到最高值。LPA干预后1h及2h时,吞噬荧光强度较对照组明显增加(P<0.01),而且吞噬率与荧光强度的乘积较对照组亦明显增加(P<0.01)。结论:LPA可提高BV2细胞活化及吞噬功能,提示LPA在小胶质细胞活化及吞噬过程中发挥了重要作用。 Objective: To observe the effect of lysophosphatidic acid (LPA) on activation and endocytosis of micro- glia. Methods: BV2 cells in exponential phase were randomly divided into two groups: control group and LPA group. At time points (1 h and 2 h), cells were harvested for later use. FCM was used to detect the phagocytic rate of FITC-dextran and the fluorescence intensity. Results: As compared with control group, the phagocytic rate of FITC-dextran in LPA group was significantly increased at 1 h (P〈0.05). At 2 h, the phagocytic rate of FITC-dex- tran reached the peak in both two groups. At time points (1 h and 2 h), as compared with control group, the fluo- rescence intensity in LPA group was increased (P〈0.01) and the product of phagocytic rate and fluorescence inten- sity in LPA group was significantly higher than that of control group (P〈0.01). Conclusion: LPA can promote ac- tivation and endocytosis of microglia in vitro, suggesting the roles of LPA in activation and endocytosis of microglia.
出处 《中国康复》 2012年第3期166-168,共3页 Chinese Journal of Rehabilitation
基金 国家自然科学基金项目(81000521 81030021)
关键词 溶血磷脂酸 小胶质细胞 FITC-葡聚糖 流式细胞术 LPA microglia FITC-dextran FCM
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