皮质酮对动物骨髓间充质干细胞迁移活性的影响及其修复学意义

Effects of corticosterone on migration activity of mesenchymal stem cells and its prosthetics significance

目的研究下丘脑-垂体-肾上腺轴效应激素———糖皮质激素(GC)对不同动物骨髓间充质干细胞迁移活性影响。方法分离、培养并鉴定大鼠和小鼠骨髓间充质干细胞,通过不同方法观察两种来源的骨髓间充质干细胞趋化活性的变化,评价GC对不同来源的骨髓间充质干细胞迁移活性的影响。结果尽管不同种属来源的骨髓间充质干细胞在形态上存在一定差异,但一定浓度的GC能够显著增强其趋化活性(P<0.05)。结论急性应激条件下,骨髓间充质干细胞迁移活性增强对损伤组织修复具有潜在的临床意义。

Objective To investigate the effects of glucocorticoid,the effector hormone of hypothalamus-pituitary-adrenal（HPA） axis on the migration activity of bone marrow derived mesenchymal stem cells in different animal species. Methods The mesenchy- mal stem cells in mice and rats were isolated,cultivated and identified. Then their chemotaxis activity was observed and the effects of glucocorticoid in these courses were detected by different methods. Results Although the mesenchymal stem cells from different animal species exhibited the difference in morphology,their chemotaxis activity was both enhanced after some concentration of glu- cocorticoid treatment. Conclusion The improvement of mesenchymal stem cells chemotaxis activity is of great importance for the repair of injured tissues after acute stress.