氧化苦参碱对LoVo细胞Bcl-2、OCLN、TUBA1A mRNA表达的影响

Effects of oxymatrine on expressions of Bcl-2,OCLN and TUBA1A in human colon carcinoma LoVo cells

目的探讨氧化苦参碱(OM)抑制人结肠癌LoVo细胞增殖和诱导凋亡的分子作用机制。方法采用实时荧光定量PCR法以及免疫组化法检测OM对LoVo细胞凋亡相关以及细胞骨架相关的B细胞淋巴瘤因子2(Bcl-2)、微管蛋白1A(TUBA1A)、咬合蛋白(OCLN)的基因及蛋白表达的影响。结果 OM能显著抑制LoVo细胞增殖;可明显抑制LoVo细胞Bcl-2的mRNA以及蛋白的表达(P<0.05),抑制TUBA1A mRNA表达,同时上调OCLN蛋白的表达,但并不能明显上调OCLN mR-NA的表达。结论 OM抑制LoVo细胞增殖,可能与下调LoVo细胞Bcl-2、TUBA1A表达以及上调OCLN表达有关。

Objective To explore the molecular mechanism of oxymatrine （OM） on inhibiting proliferation and inducing apopto- sis in human colon carcinoma LoVo cells. Methods Using fluorescence quantitative PCR and immunohistochemistry assay to detect the effects of OM on apoptosis and cytoskeleton-related molecular gene and protein expression, such as Bcl-2, OCLN, TUBA1A on LoVo cells. Results OM could inhibit LoVo cells proliferation and significantly inhibit Bcl-2 gene and protein expression on LoVo cells. OM could also significantly inhibit TUBA1A gene expression on LoVo cells. OM showed only a slight increase trend on OCLN gene expression, but with immunohistochemical assay OM could significantly increase the OCLN protein expression on LoVo cells. Conclusion The molecular mechanism of OM to inhibit tumor cell proliferation may be related to down-regulate Bcl-2 and TUBA1A expression while increased expression of OCLN on LoVo cells.