应用双向电泳和质谱联用技术研究乳源蛋白的差异性

Analysis of milk protein by two-dimensional electrophoresis combined with mass spectrometry

应用双向电泳和质谱联用技术,对不同乳源蛋白的差异性进行了研究。根据ImageMaster 2DPlatinum图像分析软件对不同乳源酪蛋白和乳清蛋白的双向电泳(2-DE)图谱进行蛋白斑点的匹配分析,获得21个存在于水牛奶中主要分布在低丰度蛋白区的酪蛋白差异蛋白点和24个存在于水牛奶中乳清蛋白差异蛋白点。这些差异蛋白点经质谱鉴定分析,得到4个属于水牛奶酪蛋白的主要组分和2个与水牛奶中酪蛋白有较高同源性的新组分,同时获得4个属于水牛奶乳清蛋白的主要组分和3个与水牛奶中乳清蛋白有较高同源性的组分。

Two-dimensional electrophoresiscombined with mass spectrometry was used for the analysis of the differences of milk proteins.21 differential proteins which mainly distributed in areas of low abundance existed only in water buffalo milk were identified according to comparing the profiles of caseins matched with software of ImageMaster 2D Platinum.And 24 differential whey proteins only in water buffalo milk were identified.Four casein fractions belonged to water buffalo milk casein and two new fractions which were high homology components with water buffalo milk casein were determined with the further analysis by mass spectrometry（MS）.Four casein fractions belonged to water buffalo milk whey and two new fractions which were high homology components with water buffalo milk whey using were also determined by the same method.