摘要
建立了HPLC-紫外检测法快速测定家蚕酪氨酸羟化酶活性的方法。采用的色谱柱为Venusil XBP C18(4.6 mm×100 mm,5μm),流动相pH为3.5,流速0.5 mL/min,紫外检测波长280nm,柱温30℃。L-多巴在0.1～100μg/mL范围内线性良好,r=0.9991(n=6),L-多巴最低检测限是0.02μg/mL(S/N≥3),日内、日间相对标准偏差(RSD)在3.1%～12%之间,样品回收率在89%～104.6%之间。该方法可用于昆虫酪氨酸羟化酶(TH)活性测定。
A method for the rapid determination of bombyx mori tyrosine hydroxylase activity by high performance liquid chromatography with UV detection was proposed.The analytical column was a Venusil XBP C18 column（4.6mm×100mm,5μm）,and the mobile phase was adjusted to pH3.5 with hydrochloric acid,the flow rate was set 0.5mL/min,UV detection was set at 280 nm,and column temperature was 30℃.The linear concentration range was 0.1~100μg/mL with good correlation coefficient of 0.9991（n=6）.The detection limit was 0.02μg/mL（S/N≥3）.The RSDs of intraday and interday were between 3.1% and 12% of middle and high concentration samples.The sample recoveries were between 89.0% and 104.6%（n=6）.The method is rapid,stable and highly sensitive.It is suitable for the determination of insect tyrosine hydroxylase activity.
出处
《分析试验室》
CAS
CSCD
北大核心
2012年第7期29-31,共3页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(81071306)资助
