摘要
目的研究肉苁蓉对运动训练大鼠睾酮含量、物质代谢及抗运动疲劳能力的影响。方法以大强度耐力训练大鼠为模型。将7周龄雄性Wistar大鼠65只为对象随机分为4组:静止ig服水组(C组)、静止ig肉苁蓉组(M组)、运动ig水组(T组)、运动ig肉苁蓉组(TM组),每组15只(剔除不符合实验要求的大鼠)。采用专业灌胃器每天ig一次,M、TM组剂量为6.01 g.kg-1,ig体积为5 mL.kg-1,C,T 2组ig等量生理盐水。42 d力竭游泳训练后,测定体重、力竭游泳时间及血睾酮等生化指标。结果 TM组体重大于T组(P<0.05),力竭游泳时间长于T组(P<0.01)。力竭游泳导致大鼠血清睾酮下降,T组与C组比较,下降了40.53%(P<0.01)。TM组与T组比较,上升了44.35%(P<0.01);血清皮质酮水平各组间均无显著差异,血清睾酮与皮质酮比值变化与睾酮变化较为一致;肝糖原、肌糖原储量下降,T组与C组比较,肝糖原下降25.85%(P<0.01),肌糖原下降44.94%(P<0.01)。TM组与T组相比,肝糖原高出19.41%(P<0.05),肌糖原高出58.05%(P<0.01);FSH无明显影响;血尿素上升,血红蛋白下降,T组与C组比较,血尿素上升了59.30%(P<0.01),血红蛋白下降28.72%(P<0.01);TM组与T组相比,血尿素低出20.70%(P<0.05),血红蛋白高出24.22%(P<0.05)。结论肉苁蓉可以减轻血睾酮受高强度运动量的影响,并维持在正常生理水平;可以促进蛋白质合成,抑制氨基酸和蛋白质分解,提高运动训练大鼠血红蛋白含量和糖原的储备。
OBJECTIVE To study the effects of Herba Cistanches on the content of testosterone, substance metabolism and the a- bility of resisting exercise-induced fatigue of rats. METHODS High-intensity endurance trained rats were used as the model. Sixty-five 7-week-old male Wistar rats were divided into 4 groups, with 15 in each group: still ig administration of water group (C group), still ig administration of Cistanehe group ( M group), exercise ig administration of water group ( T group), and exercise ig administration of Cistanche group (TM group). Gavage was done using professional device once a day. M and TM group were dosed at 6. 01 g kg- l with volume of 5 mL kg-1, while C and T group were given saline of the same volume. After 42 d exhaustive swimming training, body weight, swimming time, serum testosterone, and other biochemical markers were measured. RESULTS The body weights and swimming time of the rats in TM group were greater than those in T group ( P 〈 0. 05, P 〈 0. 01 ). Exhaustive swimming led to decreased serum testosterone. The serum testosterone levels of T group decreased by 40. 53% (P 〈 0. 01 ) compared with C group. Compared with T group, the serum testosterone levels of TM group increased by 44. 35% ( P 〈 0. 01 ). The serum corticosterone levels in each group showed no significant differences, and the serum testosterone/corticosterone ratios changed in line with changes of testosterone. Liver gly- cogen and muscle glycogen reserves failed. Compared with C group, T group's glycogen decreased by 25. 85% (P 〈 0. 01 ), and muscle glycogen decreased by 44.94% (P 〈0. 01). Compared with T group, TM group had 19.41% higher liver glycogen ( P 〈0.05 ) and 58. 05% higher muscle glycogen (P 〈 0. 01 ) ; FSH had no significant change; compared with C group, the blood urea of T group in- creased by 59. 30% ( P 〈 0. 01 ), and the hemoglobin decreased by 28. 72% ( P 〈 0. 01 ) ; compared with T group, TM group had 20. 70% lower blood urea (P 〈 0. 05) and 24. 22% higher hemoglobin (P 〈 0. 05). CONCLUSION Cistanche deserticola Y. C. Ma can reduce the impact of high-intensity exercise on serum testosterone, and maintain it at normal physiological level. It can promote protein synthesis, inhibit amino acid and protein degradation, and increase hemoglobin and glycogen reserves in rats receiving exercise training.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第13期1035-1038,共4页
Chinese Pharmaceutical Journal
基金
北京市属高等学校人才强教计划资助项目(PHR201107150)
关键词
肉苁蓉
睾酮
抗疲劳
大鼠
肝糖原
肌糖原
血尿素
血红蛋白
Herba Cistanches
testosterone
anti-fatigue
rat
liver glycogen
muscle glycogen
blood urea nitrogen
hemoglobin