信号转导抑制因子-3与磷酸化信号转导转录活化因子-3在喉癌组织中的表达及与临床病理因素的关系

Expression of SOCS-3 and p-STAT3 in laryngeal squamous cell carcinoma and the correlation with clinicopathological parameters

目的研究细胞因子信号转导抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)在喉鳞状细胞癌(LSCC)组织中的表达,并进一步探讨SOCS-3与磷酸化信号转导转录活化因子-3(phosphorylation signal transducer and activator of transcription 3,p-STAT3)的相关性及与喉癌临床病理因素的关系。方法收集2005年2月～2009年2月在白求恩国际和平医院耳鼻咽喉头颈外科接受手术的LSCC石蜡标本40例,并选取11例正常喉黏膜组织作为对照。采用免疫组化染色法检测SOCS-3、p-STAT3蛋白的表达情况。结果①LSCC组织中SCOS-3蛋白表达明显低于正常喉黏膜组织,两者之间差异具有统计学意义(P=0.000);p-STAT3蛋白在正常喉黏膜组织中的表达小于喉癌组织(P=0.000);②LSCC组织中SOCS-3与p-STAT3蛋白之间存在显著负相关(r=-0.459,P=0.003);③SOCS-3表达与患者年龄(P=0.223)、性别(P=0.849)、肿瘤T分级(P=0.193)无相关性,而与肿瘤病理分期(P=0.0 0 0),淋巴结转移(P=0.0 0 0)有关。结论①在喉正常黏膜组织中SOCS-3均呈阳性表达,而在LSCC组织中部分呈阳性表达;并与p-STAT3蛋白的表达情况呈负相关。提示SOCS-3可能在喉癌生长的过程通过抑制JAK/STAT3信号转导通路的持续活化从而抑制喉癌细胞增长,促进细胞凋亡;②SOCS-3的表达与患者年龄、性别、肿瘤T分级无相关性,而与喉癌病理分期及颈部淋巴结转移有关。

Objective To investigate the expression of suppressor of cytokine signaling-3（SOCS-3） in laryngeal squamous cell carcinoma（LSCC）,and to study the correlation between the expression of SOCS-3 and signal transducer and activator of trans p-STAT3,and that between the expression of SOCS-3 with clinicopathological factors of LSCC.Methods Immunohistochemical staining（S-P method） was used for detecting the expression of SOCS-3,p-STAT3 in 40 specimens of surgically resected LSCC and 11 of paracancerous normal laryngeal tissue.Results ① The expression of SOCS-3 in LSCC was significantly lower than that in normal laryngeal tissue（P=0.000）.The expression of p-STAT3 in normal laryngeal tissues was significantly lower than that in LSCC（P=0.000）.② Significant negative correlation was observed between the expression of SOCS-3 and that of p-STAT3（r=-0.459,P=0.003）.③ The expression of SOCS-3 was significantly related with the pathologic grading and lymphatic metastasis,but not with the age,gender,and clinical stage of the patients.Conclusion The SOCS-3 protein is omni-expressed in all normal laryngeal tissues,but less expressed in LSCC.The expression of SOCS-3 is negatively correlated to that of p-STAT3,which suggests that SOCS-3 plays an important role in inhibiting cell proliferation and promoting cell apoptosis in LSCC.The expression of SOCS-3 protein is not related to patients＇ age,gender,T-stage and tumor position,but related to cell differentiation level and lymphatic metastasis.